Laboratory of Compared Ophthalmology Investigation, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Department of Ophthalmology, School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
Vet Ophthalmol. 2020 Mar;23(2):358-367. doi: 10.1111/vop.12733. Epub 2020 Feb 12.
Optical coherence tomography (OCT) measurement of adult blue-fronted parrots (Amazona aestiva), free from infectious, inflammatory or neoplastic systemic diseases and from any ophthalmological illness, aim at its characterization, as well as to standardize the examination technique for the species.
Pupillary dilation was achieved with rocuronium bromide (5 mg/mL) at 0, 2, 15, 17, 30, and 32 minutes. The animals were sedated with midazolam maleate (0.5 mg/kg/IM) and anesthetized with propofol (5.0 mg/kg/IV). Measurements were made to evaluate the thickness of the total retina (TR), sensorineural retinal (SR), and ganglion cell complex (GCC), 2 millimeters (mm) from the pecten toward the fovea. OCT data were compared to measurements of retinal histological slides from enucleated eyes of blue-fronted parrots, scanned in automatic fluorescence microscope and measured with by the VS-ASW software.
Averages of measurements from the 43 retinas evaluated by OCT were TR: 279.40 micrometers (μm), SR: 255.90 μm, and GCC: 138.60 μm, respectively, and the measurements of six retinas using fluorescence microscopy were 260.30 μm for TR, 238.20 μm for SR, and 129.30 μm for GCC, demonstrating a high correlation coefficient between all measurements (r = .8698, P < .0001). It is also possible to evaluate the anatomy of the retina and to identify its layers, variations and abnormalities using OCT images. Variations were found between the different areas of the retina, both in the images of the histological slides and in the images of the OCT.
Optical coherence tomography is a valuable technique for in vivo evaluation of retinal structures in blue-front parrots, providing detailed and accurate images. This method improves the understanding of retinal diseases, monitoring the beginning, progression and therapy of retinal diseases, in the same individuals during longitudinal studies. In comparison to histological investigations, OCT enables imaging in vivo, therefore reducing the number of euthanized animals or enucleated eyes.
对无传染性、炎症或肿瘤性系统性疾病及任何眼疾的成年蓝额鹦鹉（Amazona aestiva）进行光学相干断层扫描（OCT）测量，旨在对其进行特征描述，并对该物种的检查技术进行标准化。
用罗库溴铵（5mg/mL）对瞳孔进行散瞳，时间分别为 0、2、15、17、30 和 32 分钟。用马来酸咪达唑仑（0.5mg/kg/IM）对动物进行镇静，并用丙泊酚（5.0mg/kg/IV）进行麻醉。测量值用于评估总视网膜（TR）、感觉神经视网膜（SR）和节细胞复合体（GCC）的厚度，从扇贝到黄斑的距离为 2 毫米（mm）。OCT 数据与蓝额鹦鹉眼窝摘除眼球的视网膜组织学切片的测量值进行比较，这些切片在自动荧光显微镜下扫描，并使用 VS-ASW 软件进行测量。
43 只眼睛通过 OCT 评估的测量值平均值分别为 TR：279.40 微米（μm）、SR：255.90μm 和 GCC：138.60μm，使用荧光显微镜测量的 6 只眼睛的测量值分别为 TR：260.30μm、SR：238.20μm 和 GCC：129.30μm，所有测量值之间的相关性很高（r=.8698，P<.0001）。OCT 图像还可用于评估视网膜的解剖结构，并识别其层、变化和异常。在组织学切片的图像和 OCT 图像中，都发现了视网膜不同区域之间的变化。
光学相干断层扫描是评估蓝额鹦鹉视网膜结构的一种有价值的技术，可提供详细、准确的图像。该方法可提高对视网膜疾病的理解，在纵向研究中，对同一个体的视网膜疾病的起始、进展和治疗进行监测。与组织学研究相比，OCT 可以进行体内成像，因此减少了需要安乐死的动物或眼窝摘除的眼睛数量。
