Arif Mokhtar Abdulsattar, Guarino Salvatore, Colazza Stefano, Peri Ezio
Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Science Ed. 5, 90128 Palermo, Italy.
Institute of Biosciences and Bioresources (IBBR), National Research Council of Italy (CNR), Corso Calatafimi 414, 90129 Palermo, Italy.
Insects. 2020 Feb 9;11(2):109. doi: 10.3390/insects11020109.
The pentatomid bug is a key pest of brassicaceous crops in several areas of the world. Previous studies suggest that mate location of this species is mediated by volatile chemicals produced by males, among which the main compound is ()-2-octenyl acetate. However, the possible attraction of males, females, and nymphs to this compound has not yet been specifically tested. In this study, we tested the response of females, males, and nymphs to ()-2-octenyl acetate using an electroantennogram (EAG) and olfactometer in the presence or absence of a host plant. Moreover, ()-2-octenyl acetate as an attractant lure in field trap bioassays was evaluated. EAG recordings showed that this compound evokes antennal responses in females. Olfactometer behavioral responses showed that females and nymphs were attracted to ()-2-octenyl acetate, while males showed no attraction. In the field trap bioassays, captures were obtained in traps baited with 5 and 10 mg of ()-2-octenyl acetate, while in traps loaded with 2 mg and control traps, there were no recorded catches. These results suggest the involvement of ()-2-octenyl acetate in intraspecific interactions of this species.
在世界上的多个地区，蝽象是十字花科作物的一种主要害虫。先前的研究表明，该物种的配偶定位是由雄性产生的挥发性化学物质介导的，其中主要化合物是（）-2-乙酸辛酯。然而，雄性、雌性和若虫对这种化合物可能的吸引力尚未经过专门测试。在本研究中，我们使用触角电图（EAG）和嗅觉仪，在有或没有寄主植物的情况下，测试了雌性、雄性和若虫对（）-2-乙酸辛酯的反应。此外，还评估了（）-2-乙酸辛酯作为田间诱捕生物测定中的引诱剂诱饵的效果。EAG记录表明，这种化合物能引起雌性的触角反应。嗅觉仪行为反应表明，雌性和若虫被（）-2-乙酸辛酯吸引，而雄性则没有表现出吸引力。在田间诱捕生物测定中，用5毫克和10毫克（）-2-乙酸辛酯诱饵的诱捕器捕获到了昆虫，而装有2毫克诱饵的诱捕器和对照诱捕器则没有记录到捕获情况。这些结果表明（）-2-乙酸辛酯参与了该物种的种内相互作用。
