费希尔344大鼠肝脏的聚尿苷酸（poly(U)）翻译能力不会随年龄增长而退化，且不受饮食方式的影响。

The poly(U) translational capacity of Fischer 344 rat liver does not deteriorate with age and is not affected by dietary regime.

作者信息

Laughrea M, Latulippe J

机构信息

Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Québec, Canada.

出版信息

Mech Ageing Dev. 1988 Nov 14;45(2):137-43. doi: 10.1016/0047-6374(88)90103-0.

DOI:10.1016/0047-6374(88)90103-0

PMID:3205068

Abstract

The translational capacity of ribosomes isolated from the livers of young (60-170 days, n = 13) and old (26.5 months, n = 11) Fischer 344 rats was assessed by poly(U)-directed [3H]phenylalanine incorporation. Run-off ribosomes and S100 supernatant were separately prepared from each individual liver. Ribosome concentrations were carefully measured. Individual differences were seen among young rats and among old rats; however, when considered as a group, the old rats, whether diet-restricted or fed ad libitum, translated poly(U) as well, or almost as well, as the young ones.

摘要

通过聚（U）指导的[3H]苯丙氨酸掺入来评估从年轻（60 - 170天，n = 13）和年老（26.5个月，n = 11）的Fischer 344大鼠肝脏中分离出的核糖体的翻译能力。从每只大鼠的肝脏中分别制备延伸核糖体和S100上清液。仔细测量核糖体浓度。在年轻大鼠之间和年老大鼠之间均观察到个体差异；然而，作为一个整体来看，无论饮食受限还是自由进食的年老大鼠，其翻译聚（U）的能力与年轻大鼠一样好，或者几乎一样好。