蓖麻毒素对蛋白质合成的抑制作用：大鼠肝脏线粒体与细胞核以及大肠杆菌核糖体的实验
Inhibition of protein synthesis by ricin: experiments with rat liver mitochondria and nuclei and with ribosomes from Escherichia coli.
作者信息
Greco M, Montanaro L, Novello F, Saccone C, Sperti S, Stirpe P
出版信息
Biochem J. 1974 Sep;142(3):695-7. doi: 10.1042/bj1420695.
Abstract
- Ricin, a toxic protein from the seeds of Ricinus communis which inhibits poly(U)-directed polyphenylalanine synthesis by rat liver ribosomes (Montanaro et al., 1973), does not affect protein synthesis by isolated rat liver mitochondria. 2. The toxin is ineffective also on poly(U)-directed polyphenylalanine synthesis in reconstituted systems with ribosomes isolated from rat liver mitochondria or from Escherichia coli. 3. Ricin inhibits protein synthesis by isolated rat liver nuclei, but at concentrations much higher than those affecting rat liver ribosomes.
摘要
- 蓖麻毒素是一种来自蓖麻种子的有毒蛋白质，它能抑制大鼠肝脏核糖体的多聚尿苷酸（poly(U)）指导的聚苯丙氨酸合成（蒙塔纳罗等人，1973年），但不影响分离的大鼠肝脏线粒体的蛋白质合成。2. 该毒素对用从大鼠肝脏线粒体或大肠杆菌分离的核糖体重建的系统中的多聚尿苷酸指导的聚苯丙氨酸合成也无效。3. 蓖麻毒素抑制分离的大鼠肝脏细胞核的蛋白质合成，但所需浓度远高于影响大鼠肝脏核糖体的浓度。
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本文引用的文献
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The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides.大肠杆菌中无细胞蛋白质合成对天然存在或合成的多聚核糖核苷酸的依赖性。
Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1588-602. doi: 10.1073/pnas.47.10.1588.
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The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by a soluble protein-ribosome system from Escherichia coli.酶促合成的核糖核酸对大肠杆菌可溶性蛋白质 - 核糖体系统掺入氨基酸的影响。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):94-104. doi: 10.1073/pnas.48.1.94.
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Ricin - a potent inhibitor of protein synthesis.蓖麻毒素——一种强大的蛋白质合成抑制剂。
FEBS Lett. 1972 Feb 15;20(3):327-329. doi: 10.1016/0014-5793(72)80098-x.
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RNA synthesis in isolated rat liver mitochondria.大鼠肝脏分离线粒体中的RNA合成
Eur J Biochem. 1969 Aug;10(1):61-5. doi: 10.1111/j.1432-1033.1969.tb00655.x.
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Separation of mitochondrial and cytoplasmic peptide chain elongation factors from yeast.酵母线粒体和细胞质肽链延长因子的分离
Biochemistry. 1970 Dec 22;9(26):5065-70. doi: 10.1021/bi00828a004.
6
Synthetic activity of isolated rat liver nuclei. II. Evidence for protein formation.分离的大鼠肝细胞核的合成活性。II. 蛋白质形成的证据。
Exp Cell Res. 1973 Jun;79(2):391-403. doi: 10.1016/0014-4827(73)90459-x.
7
Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用。核糖体作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):677-83. doi: 10.1042/bj1360677.
8
Isolation and characterization of rat-liver mitochondrial ribosomes highly active in poly(U)-directed polyphenylalanine synthesis.
Eur J Biochem. 1973 Aug 1;37(1):171-7. doi: 10.1111/j.1432-1033.1973.tb02972.x.
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