蓖麻毒素对蛋白质合成的抑制作用：大鼠肝脏线粒体与细胞核以及大肠杆菌核糖体的实验
Inhibition of protein synthesis by ricin: experiments with rat liver mitochondria and nuclei and with ribosomes from Escherichia coli.
作者信息
Greco M, Montanaro L, Novello F, Saccone C, Sperti S, Stirpe P
出版信息
Biochem J. 1974 Sep;142(3):695-7. doi: 10.1042/bj1420695.
Abstract
- Ricin, a toxic protein from the seeds of Ricinus communis which inhibits poly(U)-directed polyphenylalanine synthesis by rat liver ribosomes (Montanaro et al., 1973), does not affect protein synthesis by isolated rat liver mitochondria. 2. The toxin is ineffective also on poly(U)-directed polyphenylalanine synthesis in reconstituted systems with ribosomes isolated from rat liver mitochondria or from Escherichia coli. 3. Ricin inhibits protein synthesis by isolated rat liver nuclei, but at concentrations much higher than those affecting rat liver ribosomes.
摘要
- 蓖麻毒素是一种来自蓖麻种子的有毒蛋白质，它能抑制大鼠肝脏核糖体的多聚尿苷酸（poly(U)）指导的聚苯丙氨酸合成（蒙塔纳罗等人，1973年），但不影响分离的大鼠肝脏线粒体的蛋白质合成。2. 该毒素对用从大鼠肝脏线粒体或大肠杆菌分离的核糖体重建的系统中的多聚尿苷酸指导的聚苯丙氨酸合成也无效。3. 蓖麻毒素抑制分离的大鼠肝脏细胞核的蛋白质合成，但所需浓度远高于影响大鼠肝脏核糖体的浓度。
相似文献
1
Inhibition of protein synthesis by ricin: experiments with rat liver mitochondria and nuclei and with ribosomes from Escherichia coli.蓖麻毒素对蛋白质合成的抑制作用：大鼠肝脏线粒体与细胞核以及大肠杆菌核糖体的实验
Biochem J. 1974 Sep;142(3):695-7. doi: 10.1042/bj1420695.
2
Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用：60S核糖体亚基作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):813-5. doi: 10.1042/bj1360813.
3
Differential ricin sensitivity of rat liver and wheat germ ribosomes in polyuridylic acid translation.大鼠肝脏核糖体和小麦胚芽核糖体在多聚尿苷酸翻译中的蓖麻毒素敏感性差异
Arch Biochem Biophys. 1977 Aug;182(2):690-5. doi: 10.1016/0003-9861(77)90550-1.
4
Inhibition of Escherichia coli protein synthesis by a limited tryptic digest of ricin, the toxin of Ricinus communis L. seeds.
Biochem Biophys Res Commun. 1980 Dec 16;97(3):962-7. doi: 10.1016/0006-291x(80)91470-9.
5
Action of ricin from Ricinus communis L. seeds on eukaryote ribosomal proteins.蓖麻（Ricinus communis L.）种子中的蓖麻毒素对真核生物核糖体蛋白的作用。
FEBS Lett. 1976 Sep 1;67(3):343-7. doi: 10.1016/0014-5793(76)80561-3.
6
Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用。核糖体作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):677-83. doi: 10.1042/bj1360677.
7
Protection of rat liver 80 S ribosomes against ricin A chain inactivation by proteins extracted from rat liver and wheat germ ribosomal subunits with ammonium chloride/magnesium chloride.用氯化铵/氯化镁从大鼠肝脏和小麦胚芽核糖体亚基中提取的蛋白质对大鼠肝脏80S核糖体的保护作用，防止蓖麻毒素A链失活
Biochim Biophys Acta. 1981 Sep 28;655(2):230-42. doi: 10.1016/0005-2787(81)90013-7.
8
Correlation of inhibition of ribosomal GTPase and protein synthesis by abrin and ricin.相思子毒素和蓖麻毒素对核糖体GTP酶的抑制作用与蛋白质合成的相关性。
Biochem Biophys Res Commun. 1977 Apr 25;75(4):845-50. doi: 10.1016/0006-291x(77)91459-0.
9
Studies on the accessability of ribosomes to inactivation by the toxic lectins abrin and ricin.关于核糖体对毒性凝集素相思子毒素和蓖麻毒素失活作用的易感性研究。
Eur J Biochem. 1977 Apr 1;74(2):209-15. doi: 10.1111/j.1432-1033.1977.tb11383.x.
10
Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.核糖体失活蛋白对大肠杆菌和根癌土壤杆菌翻译系统的影响。
J Bacteriol. 1993 Oct;175(20):6721-4. doi: 10.1128/jb.175.20.6721-6724.1993.
引用本文的文献
1
Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.核糖体失活蛋白对大肠杆菌和根癌土壤杆菌翻译系统的影响。
J Bacteriol. 1993 Oct;175(20):6721-4. doi: 10.1128/jb.175.20.6721-6724.1993.
2
Ribosomal core-particles as the target of ricin.核糖体核心颗粒作为蓖麻毒素的靶点。
Biochem J. 1981 Mar 15;194(3):1015-7. doi: 10.1042/bj1941015.
3
[Biology of lectins and their application in clinical biochemistry (author's transl)].[凝集素的生物学及其在临床生物化学中的应用（作者译）]
Klin Wochenschr. 1977 Apr 15;55(8):359-73. doi: 10.1007/BF01488621.
本文引用的文献
1
The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides.大肠杆菌中无细胞蛋白质合成对天然存在或合成的多聚核糖核苷酸的依赖性。
Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1588-602. doi: 10.1073/pnas.47.10.1588.
2
The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by a soluble protein-ribosome system from Escherichia coli.酶促合成的核糖核酸对大肠杆菌可溶性蛋白质 - 核糖体系统掺入氨基酸的影响。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):94-104. doi: 10.1073/pnas.48.1.94.
3
Ricin - a potent inhibitor of protein synthesis.蓖麻毒素——一种强大的蛋白质合成抑制剂。
FEBS Lett. 1972 Feb 15;20(3):327-329. doi: 10.1016/0014-5793(72)80098-x.
4
RNA synthesis in isolated rat liver mitochondria.大鼠肝脏分离线粒体中的RNA合成
Eur J Biochem. 1969 Aug;10(1):61-5. doi: 10.1111/j.1432-1033.1969.tb00655.x.
5
Separation of mitochondrial and cytoplasmic peptide chain elongation factors from yeast.酵母线粒体和细胞质肽链延长因子的分离
Biochemistry. 1970 Dec 22;9(26):5065-70. doi: 10.1021/bi00828a004.
6
Synthetic activity of isolated rat liver nuclei. II. Evidence for protein formation.分离的大鼠肝细胞核的合成活性。II. 蛋白质形成的证据。
Exp Cell Res. 1973 Jun;79(2):391-403. doi: 10.1016/0014-4827(73)90459-x.
7
Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用。核糖体作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):677-83. doi: 10.1042/bj1360677.
8
Isolation and characterization of rat-liver mitochondrial ribosomes highly active in poly(U)-directed polyphenylalanine synthesis.
Eur J Biochem. 1973 Aug 1;37(1):171-7. doi: 10.1111/j.1432-1033.1973.tb02972.x.
9
Synthetic activity of isolated rat liver nuclei. I. Ultrastructural study at various steps of isolation.大鼠离体肝细胞核的合成活性。I. 分离各步骤的超微结构研究。
Exp Cell Res. 1973 Feb;76(2):337-48. doi: 10.1016/0014-4827(73)90385-6.
10
Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用：60S核糖体亚基作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):813-5. doi: 10.1042/bj1360813.
Zotero 插件