Kudo Nobuhiro, Kimura Yasuo
Division of Plant Biotechnology, Gunma Horticultural Experiment Station, 493 Nishi-Obokata, Sawa-Azuma, Gunma, 379-2224, Japan.
Ann Bot. 2001 Feb 1;87(2):275-281. doi: 10.1006/anbo.2000.1337.
Flow cytometric analysis of nuclear DNA contents of somatic tissues of cabbage ( Brassica oleracea L.) has revealed extensive endopolyploidization, resulting in tissues that comprise mixtures of cells with different DNA contents, ranging from 2C to 16C. Patterns of endopolyploidy are specific to each developmental stage. Multiple polyploidy was not present in the embryos of dry seeds. Rapid endoreduplication occurred in the radicle and the hypocotyl of the embryos during seed germination. Further endoreduplication cycles were detected in all tissues except those of the shoot tips. In five cabbage cultivars tested, seedlings contained cells of four ploidy levels, corresponding to 2C, 4C, 8C and 16C. Multiploidy may be an integral part of differentiation programs in cabbage plants. The biological significance of endoreduplication in cabbage plants is discussed.
对甘蓝（Brassica oleracea L.）体细胞组织的核DNA含量进行流式细胞术分析，结果显示存在广泛的核内多倍化现象，导致组织中包含不同DNA含量的细胞混合物，范围从2C到16C。核内多倍化模式在每个发育阶段都是特定的。干燥种子的胚中不存在多倍体。种子萌发过程中，胚的胚根和下胚轴发生快速核内复制。除茎尖组织外，在所有组织中都检测到了进一步的核内复制周期。在测试的五个甘蓝品种中，幼苗含有四个倍性水平的细胞，分别对应2C、4C、8C和16C。多倍体可能是甘蓝植物分化程序的一个组成部分。本文讨论了甘蓝植物核内复制的生物学意义。
