咖啡因和绿原酸对碘化丙啶与DNA结合能力的影响：对咖啡树基因组大小评估的影响
Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree.
作者信息
Noirot M, Barre P, Duperray C, Louarn J, Hamon S
机构信息
IRD BP5045, 34032 Montpellier Cedex, France.
出版信息
Ann Bot. 2003 Aug;92(2):259-64. doi: 10.1093/aob/mcg139.
Abstract
Estimates of genome size using flow cytometry can be biased by the presence of cytosolic compounds, leading to pseudo-intraspecific variation in genome size. Two important compounds present in coffee trees-caffeine and chlorogenic acid-modify accessibility of the dye propidium iodide to Petunia DNA, a species used as internal standard in our genome size evaluation. These compounds could be responsible for intraspecific variation in genome size since their contents vary between trees. They could also be implicated in environmental variations in genome size, such as those revealed when comparing the results of evaluations carried out on different dates on several genotypes.
摘要
使用流式细胞术估计基因组大小可能会因胞质化合物的存在而产生偏差，从而导致基因组大小出现假种内变异。咖啡树中存在的两种重要化合物——咖啡因和绿原酸——会改变碘化丙啶染料对矮牵牛DNA的可及性，矮牵牛DNA是我们在基因组大小评估中用作内标的物种。由于这些化合物在不同树木间的含量不同，它们可能是造成基因组大小种内变异的原因。它们也可能与基因组大小的环境变异有关，比如在比较不同日期对几种基因型进行评估的结果时所揭示的变异。
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