Department of Biochemistry, Hebrew University Faculty of Medicine, Jerusalem, Israel.
Department of Hematology, Hadassah-Hebrew University Hospital, Jerusalem, Israel.
Transfus Apher Sci. 2020 Jun;59(3):102738. doi: 10.1016/j.transci.2020.102738. Epub 2020 Feb 5.
Donated blood is stored in the blood bank as packed red blood cell units. In the process of packed cells preparation, the red blood cells (RBCs) are subjectedto high level of shear stress, which can induce alterations in their properties. In the present study, we examined the effect of packed RBCs preparation (which included leuko-filtration) on red cell deformability.
Blood samples were collected from 25 healthy donors and from corresponding units of packed RBCs. The portion of undeformable cells (%UDFC) was determined for each sample.
The median value of %UDFC was equal to 6.75 ± 0.70 %, for freshly-donated RBCs, and to 6.36 ± 0.51 %, for packed cells. Wherein, %UDFC may increase or decrease following packed cells preparation, depending upon the initial portion of undeformable cells.
Likely, exposure of RBCs to high shear stress, during packed cells preparation, induces opposing effects: (a) removal/destruction of rigid (undeformable) cells, thereby reducing their total amount (i.e., decreasing the %UDFC) on the one hand, and (b) mechanical damage to the cell membrane and subsequent reduction of the cell deformability (thereby increasing the %UDFC) on the other. As a consequence, the final impact of packed cells preparation is primarily determined by the initial state of erythrocytes in the blood of the donor.
捐献的血液以浓缩红细胞单位的形式储存在血库中。在浓缩红细胞制备过程中，红细胞（RBC）会受到高水平的剪切力，这可能导致其性质发生变化。在本研究中，我们研究了浓缩红细胞制备（包括白细胞滤除）对红细胞变形能力的影响。
从 25 名健康供体和相应的浓缩红细胞单位中采集血样。确定每个样本的不可变形细胞的比例（%UDFC）。
新鲜捐献的 RBC 的中位数%UDFC 值为 6.75±0.70%，浓缩红细胞的中位数%UDFC 值为 6.36±0.51%。其中，浓缩红细胞制备后%UDFC 值可能会增加或减少，这取决于初始不可变形细胞的比例。
红细胞在浓缩红细胞制备过程中暴露于高剪切力可能会产生相反的影响：（a）刚性（不可变形）细胞的去除/破坏，从而减少其总量（即%UDFC 值降低），另一方面，（b）细胞膜的机械损伤和随后的细胞变形能力降低（即%UDFC 值升高）。因此，浓缩红细胞制备的最终影响主要取决于供体血液中红细胞的初始状态。
