Gitanjli Gitanjli, Sankhyan Varun, Thakur Y P, Dogra P K
Animal Genetics and Breeding, COVAS, CSKHPKV, Palampur, 176062, India.
Livestock Production Management, COVAS, CSKHPKV, Palampur, 176062, India.
Trop Anim Health Prod. 2020 Jul;52(4):2091-2099. doi: 10.1007/s11250-020-02227-4. Epub 2020 Feb 12.
Goat production under migratory system is foremost meat resource in Western Himalayan region of India. Thus, selection of goats for superior growth rate is rewarding. Growth hormone (GH) gene is identified as main regulator of post-natal growth and development. The objective of this study was to identify GH gene variants in Gaddi goats reared under migratory system via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-strand conformation polymorphism (SSCP). Blood samples from 63 animals from different migratory flocks registered under All India Coordinated Research Project, Himachal Pradesh Agricultural University (HPAU), Palampur, were subjected to DNA isolation. A total of 422, 116, 389 and 181-bp amplicons were generated on amplification of four targeted regions of GH gene. GH1 and GH2 fragments were analysed using PCR-RFLP (HaeIII RE) that revealed three variants (AA, AB and BB) for GH1 having frequency as 0.27, 0.52 and 0.31, respectively whereas, two variants (AB and BB) were revealed for GH2 fragment having frequency of 0.24 and 076, respectively. GH3 and GH4 fragments were subjected to PCR-SSCP that detected three genotypes (AB, BB and AA) for GH3 with respective genotype frequency as 0.57, 0.21 and 0.22 respectively; however, GH4 was found to be monomorphic. The polymorphism information content values for GH1, GH2 and GH3 were 0.37, 0.36 and 0.34, respectively, which suggested the median level of polymorphism at studied loci and also indicated the effectiveness of the studied marker for population genetic studies. Significant associations (P ≤ 0.05) were detected for GH1 with 9-month body weight, GH2 with 9 and 12-month heart girth and GH3 with 6-month body weight, body height and body length, respectively. From the present study, it was concluded that SNPs and their association with some body measurements may be employed as useful markers for ongoing phenotypic selection programme.
在游牧系统下的山羊养殖是印度西喜马拉雅地区最重要的肉类资源。因此，选择生长速度快的山羊是有意义的。生长激素（GH）基因被确定为出生后生长发育的主要调节因子。本研究的目的是通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和单链构象多态性（SSCP）来鉴定在游牧系统下饲养的加迪山羊的GH基因变异。从印度喜马偕尔邦农业大学（HPAU）帕拉姆布尔分校全印度协调研究项目登记的不同游牧群体的63只动物采集血样，进行DNA分离。对GH基因的四个靶向区域进行扩增，共产生了422、116、389和181bp的扩增子。使用PCR-RFLP（HaeIII RE）分析GH1和GH2片段，结果显示GH1有三种变异体（AA、AB和BB），频率分别为0.27、0.52和0.31；而GH2片段有两种变异体（AB和BB），频率分别为0.24和0.76。对GH3和GH4片段进行PCR-SSCP分析，检测到GH3有三种基因型（AB、BB和AA），基因型频率分别为0.57、0.21和0.22；然而，发现GH4是单态的。GH1、GH2和GH3的多态信息含量值分别为0.37、0.36和0.34，这表明在所研究的位点上多态性处于中等水平，也表明所研究的标记对群体遗传学研究是有效的。分别检测到GH1与9个月龄体重、GH2与9个月龄和12个月龄胸围、GH3与6个月龄体重、体高和体长之间存在显著关联（P≤0.05）。从本研究得出结论，单核苷酸多态性及其与一些身体测量值的关联可作为正在进行的表型选择计划的有用标记。
