Moawad Mahmoud A, Aboshady Hadeer M, Abd-Alla Mamdouh S, Ghanem Nasser, Abdel-Moneim Ahmed Y, Nishibori Masahide, Yonezawa Takahiro, Mannen Hideyuki, Agamy Rania
Animal Production Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt.
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
Trop Anim Health Prod. 2025 Jan 6;57(1):18. doi: 10.1007/s11250-024-04253-y.
Sustainable agriculture approaches necessitate a concerted effort from researchers to establish paths that meet global population needs without compromising environmental resources. Goats are unique among ruminants because of their ability to adapt to some of the harshest environments around the world. Growth Hormone (GH) gene is a major regulator of muscle mass growth. Therefore, our study aims to analyze the whole sequence of GH gene in order to identify various single nucleotide polymorphisms (SNPs) in Damascus and Black Bengal goats (BBG) and to predict the effect of mutant residues on the protein's structure and function. Additionally, this study examined the effects of these SNPs on growth characteristics in Damascus goats. The study was designed to test 22 male goats. To obtain high-quality amplification of the GH gene, we utilized the nested PCR technique and performed paired-end sequencing for each fragment. Sequences alignment in the GH gene of Damascus and BBG goats unveiled 25 SNPs with different frequencies within the two breeds. Seven SNPs identified in coding regions comprised four synonymous variants (719 C → T, 731 G → A, 1610 C → T and 1625 C → T) and three non-synonymous variants (1112 G → A, 1459 C → T and 1470 C → T). The SNP 1112 G → A led to the substitution of Glutamic acid with Lysine (E/K) at amino acid position 137. The SNP 1459 C → T led to the substitution of Alanine with Valine (A/V) at amino acid position 160. Meanwhile, the SNP 1470 C → T led to the substitution of Leucine with Phenylalanine(L/F) at amino acid position 164. The SNP 1112 G → A was predicted to have a deleterious effect on the protein's structure and function. The SNPs 914 G → A and 1112 G → A showed significant differences (P < 0.05) between genotypes in pre-slaughter weight and almost carcass cuts in Damascus goats, indicating their possible use in breeding programs as a potential genetic marker for weight/size in goats as well as in assessing and choosing members of this breed for meat production.
可持续农业方法需要研究人员共同努力，建立既能满足全球人口需求又不损害环境资源的途径。山羊在反刍动物中独具特色，因为它们有能力适应世界上一些最恶劣的环境。生长激素（GH）基因是肌肉质量生长的主要调节因子。因此，我们的研究旨在分析GH基因的全序列，以鉴定大马士革山羊和黑孟加拉山羊（BBG）中的各种单核苷酸多态性（SNP），并预测突变残基对蛋白质结构和功能的影响。此外，本研究还考察了这些SNP对大马士革山羊生长特性的影响。该研究设计测试22只雄性山羊。为了获得GH基因的高质量扩增，我们采用了巢式PCR技术，并对每个片段进行了双末端测序。大马士革山羊和BBG山羊GH基因的序列比对揭示了两个品种中频率不同的25个SNP。在编码区鉴定出的7个SNP包括4个同义变体（719 C→T、731 G→A、1610 C→T和1625 C→T）和3个非同义变体（1112 G→A、1459 C→T和1470 C→T）。SNP 1112 G→A导致第137位氨基酸处的谷氨酸被赖氨酸（E/K）取代。SNP 1459 C→T导致第160位氨基酸处的丙氨酸被缬氨酸（A/V）取代。同时，SNP 1470 C→T导致第164位氨基酸处的亮氨酸被苯丙氨酸（L/F）取代。预测SNP 1112 G→A对蛋白质的结构和功能有有害影响。SNP 914 G→A和1112 G→A在大马士革山羊的屠宰前体重和几乎所有胴体切块的基因型之间显示出显著差异（P<0.05），表明它们可能在育种计划中用作山羊体重/体型的潜在遗传标记，以及用于评估和选择该品种用于肉类生产的个体。
