Lin S Y, Lee T C, Cheng C S, Wang T C
Institute of Zoology, Academia Sinica, Taipei, Republic of China.
Mutat Res. 1988 Dec;206(4):439-45. doi: 10.1016/0165-1218(88)90051-1.
2,2-Dichlorovinyl-O,O-dimethyl phosphate (DDVP), an extensively used household insecticide, was assayed for its genotoxicity in primary rat tracheal epithelial (RTE) cells. Cytotoxicity of DDVP to RTE cells was dose-dependent, killing about 50% of the cell population at a dose of 80 micrograms/ml. Sister-chromatid exchange (SCE) and chromosomal aberrations induced by this insecticide were positive in RTE cells although the doses needed for significant inductions were much higher than those by a known genotoxic agent, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The level of transformation induced by DDVP in RTE cells was about 1/5 that induced by MNNG at a dose of similar cytotoxicity. The slope of the regression line for induced transformation is 1.27. DDVP probably induces the genotoxic effect in RTE cells by a one-hit mechanism.
2,2-二氯乙烯基-O,O-二甲基磷酸酯（敌敌畏）是一种广泛使用的家用杀虫剂，对原代大鼠气管上皮（RTE）细胞的遗传毒性进行了测定。敌敌畏对RTE细胞的细胞毒性呈剂量依赖性，在剂量为80微克/毫升时可杀死约50%的细胞群体。尽管该杀虫剂诱导显著变化所需的剂量远高于已知的遗传毒性剂N-甲基-N'-硝基-N-亚硝基胍（MNNG），但它诱导RTE细胞发生的姐妹染色单体交换（SCE）和染色体畸变呈阳性。在细胞毒性相似的剂量下，敌敌畏在RTE细胞中诱导的转化水平约为MNNG诱导水平的1/5。诱导转化的回归线斜率为1.27。敌敌畏可能通过一次打击机制在RTE细胞中诱导遗传毒性效应。
