Popescu N C, Amsbaugh S C, DiPaolo J A
Int J Cancer. 1981 Jul 15;28(1):71-7. doi: 10.1002/ijc.2910280113.
Sister chromatid exchange (SCE) and morphological transformation induced by five chemical carcinogens, N-acetoxy-2-fluorenyl-acetamide (AcAAF), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), methyl methanesulfonate (MMS), benzo[a]-pyrene (BP), and cisplatinum(II) diamine dichloride (PT) as well as by X-irradiation were quantified in parallel experiments with cultures of Syrian hamster embryo cells (HEC). Incubation of HEC with 5-bromodeoxyuridine (10(-5)M) for two rounds of replication (24 h) required for SCE visualization neither caused morphological transformation nor altered the transformation frequency induced by carcinogen alone. All chemical carcinogens, but not X-irradiation, produced a dose-dependent increase in SCE and transformation frequency, demonstrating the sensitivity of both assays to carcinogens. The ratios of induced SCE relative to transformation frequency, however, varied with the carcinogen. BP, MMNG, and AcAAF were similarly efficient in inducing SCE compared to transformation but were considerably less effective than MMS and PT. X-irradiation at doses of 200, 300, and 500 R did not cause transformation and induced a low frequency of SCE. On a molar basis, MMS and PT were the most effective in SCE induction relative to transformation, MNNG and AcAAF were less effective, and BP was the least effective carcinogen. The positive linear correlation between chemical carcinogen-induced SCE and transformation suggests a relationship between the two cellular responses.
在叙利亚仓鼠胚胎细胞（HEC）培养物的平行实验中，对由五种化学致癌物，即N - 乙酰氧基 - 2 - 芴基乙酰胺（AcAAF）、N - 甲基 - N'- 硝基 - N - 亚硝基胍（MNNG）、甲磺酸甲酯（MMS）、苯并[a]芘（BP）和顺铂（II）二胺二氯化物（PT）以及X射线诱导的姐妹染色单体交换（SCE）和形态转化进行了定量分析。用5 - 溴脱氧尿苷（10⁻⁵M）孵育HEC进行两轮复制（24小时）以观察SCE，这既未引起形态转化，也未改变单独由致癌物诱导的转化频率。所有化学致癌物，但X射线除外，均使SCE和转化频率呈剂量依赖性增加，表明这两种检测方法对致癌物均敏感。然而，诱导的SCE与转化频率的比值因致癌物而异。与转化相比，BP、MMNG和AcAAF在诱导SCE方面效率相似，但比MMS和PT的效果要差得多。200、300和500伦琴剂量的X射线未引起转化，且诱导的SCE频率较低。以摩尔为基础，相对于转化，MMS和PT在诱导SCE方面最有效，MNNG和AcAAF效果较差，而BP是最无效的致癌物。化学致癌物诱导的SCE与转化之间的正线性相关表明这两种细胞反应之间存在关联。
