Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, P.R. China.
Genome. 2020 Apr;63(4):239-251. doi: 10.1139/gen-2019-0160. Epub 2020 Feb 13.
Segregation distortion is a common phenomenon that has been observed in genetics and plant breeding; however, the mechanism of segregation distortion is unknown. In the present study, three half-sib F populations derived from three overwinter (perennial) rice varieties (W1, W2, and W3) crossed to the rice variety Minghui725 (MH725) were developed to construct three half-sib linkage maps. We established linkage map lengths of 2032.8, 2317.4, and 2108.7 cM with average intervals of 20.1, 20.5, and 19.7 cM using 101, 113, and 107 SSR markers in W1/MH725, W2/MH725, and W3/MH725, respectively. Discrepancies in marker order and genetic linkage distance occurred in the three half-sib linkage maps due to segregation distortion. A total of 88 markers exhibited segregation distortion across the three linkage maps at < 0.01 level, 42 segregation distortion loci (SDLs) were detected across the three half-sib populations and exhibited variable LOD value that ranged from 3.2 () to 30.1 (), and 13 of the 42 SDLs were repeatedly located at the same chromosomal regions of the previously published hybrid sterility quantitative trait loci. Data from this study provide an extensive archive for investigating the genetic characteristic of overwintering cultivated rice and the future exploration and innovation of overwintering rice breeding.
分离失真（Segregation distortion）是一种在遗传学和植物育种中经常观察到的现象，但分离失真的机制尚不清楚。本研究以三个冬性（多年生）水稻品种（W1、W2 和 W3）与明恢 725（MH725）杂交构建的三个半同胞 F 群体为材料，构建了三个半同胞连锁图谱。我们使用 101、113 和 107 个 SSR 标记，在 W1/MH725、W2/MH725 和 W3/MH725 中分别建立了长度为 2032.8、2317.4 和 2108.7 cM 的连锁图谱，平均间隔为 20.1、20.5 和 19.7 cM。由于分离失真，三个半同胞连锁图谱中标记顺序和遗传连锁距离存在差异。在三个连锁图谱中，共有 88 个标记在 < 0.01 水平上表现出分离失真，在三个半同胞群体中检测到 42 个分离失真位点（SDLs），其 LOD 值变化范围为 3.2（）到 30.1（），其中 13 个 SDLs重复定位在先前报道的杂种不育数量性状位点的相同染色体区域。本研究的数据为研究冬性栽培稻的遗传特性以及冬性稻种的未来探索和创新提供了广泛的资源。
