突尼斯的人类流产与感染：人型支原体的作用及瓦氏菌高血清阳性率

Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence.

作者信息

Smaoui Mariem, Carole Kebbi, Sellami Hanen, Kammoun Salma, Choura Khaled, Maazoun Leila, Mestiri Houssem, Aeby Sebastien, Louati Doulira, Derbel Mohamed, Chaabene Kais, Hammami Adnene, Greub Gilbert, Znazen Abir

机构信息

Laboratory of Microbiology, Research Laboratory "MPH", Habib Bourguiba University Hopsital of Sfax, Sfax University, Sfax, Tunisia.

Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University of Lausanne and University Hospital of Lausanne, Lausanne, Switzerland.

出版信息

J Infect Dev Ctries. 2019 May 31;13(5):410-418. doi: 10.3855/jidc.9829.

DOI:10.3855/jidc.9829

PMID:32053510

Abstract

INTRODUCTION

Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens.

METHODOLOGY

Cervicovaginal swabs and placenta samples from 132 women with miscarriage (patient group: PG), and cervicovaginal swabs from 54 women with normal pregnancy (control group:CG), were subjected to bacteriological culture and real time PCRs detecting Coxiella burnetii, Brucella spp, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Chlamydia trachomatis, Waddlia chondrophila and Parachlamydia acanthamoebeae DNA. Serology of C. burnetii, C. trachomatis and W. chondrophila was also performed.

RESULTS

Placenta samples were positive for E. coli, S. agalactiae, U. urealyticum, M. hominis and C. trachomatis in 4.7%, 3.1%, 3.1%, 0.7% and 0.7% of cases, respectively. For cervicovaginal swabs, M. hominis was more frequently detected among PG than CG with a significant statistical difference (p = 0.02). C. trachomatis was detected in 3.3% and 5.5% among PG and CG, respectively. U. urealyticum DNA was detected with high percentages in the two groups. Samples from both groups showed negatives results for C. burnetii, Waddlia, and Brucella qPCRs. A high rate of W. chondrophila seroprevalence (42%) was noted with significant difference among women with early miscarriage.

CONCLUSIONS

C. trachomatis, S. agalactiae and M. hominis may play a role in miscarriage. However, the full characterization of the vaginal flora using other technologies such as NGS-based metagenomics is needed to clarify their role in miscarriage. Finally, further investigations should be performed to explain high W. chondrophila seroprevalence.

摘要

引言

流产是最常见的不良妊娠结局之一。本研究的目的是调查人类流产与动物源性细菌和生殖道病原体感染之间的关系。

方法

对132例流产妇女的宫颈阴道拭子和胎盘样本（患者组：PG）以及54例正常妊娠妇女的宫颈阴道拭子（对照组：CG）进行细菌培养和实时PCR，检测伯氏考克斯体、布鲁氏菌属、人型支原体、生殖支原体、解脲脲原体、沙眼衣原体、嗜软骨沃氏菌和棘阿米巴副衣原体DNA。还进行了伯氏考克斯体、沙眼衣原体和嗜软骨沃氏菌的血清学检测。

结果

胎盘样本中，大肠杆菌、无乳链球菌、解脲脲原体、人型支原体和沙眼衣原体的阳性率分别为4.7%、3.1%、3.1%、0.7%和0.7%。对于宫颈阴道拭子，患者组中人型支原体的检出频率高于对照组，差异有统计学意义（p = 0.02）。沙眼衣原体在患者组和对照组中的检出率分别为3.3%和5.5%。两组中解脲脲原体DNA的检出率均较高。两组样本的伯氏考克斯体、沃氏菌和布鲁氏菌qPCR检测结果均为阴性。早期流产妇女中嗜软骨沃氏菌血清阳性率较高（42%），差异有统计学意义。