Borges Karen Apellanis, Martelo Eduarda Boff, Dos Santos Lilian Andriva, Furian Thales Quedi, Cisco Isabel C, Manto Luciane, Dos Santos Luciana R
Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, RS, Brazil.
J Infect Dev Ctries. 2019 May 31;13(5):455-460. doi: 10.3855/jidc.11107.
Salmonella is a major cause of foodborne illness throughout the world. The use of quantitative techniques is important for assessing the risk and determining the capacity of each step of the slaughtering process to decrease or increase bacterial contamination. We aimed to detect and to quantify the presence of Salmonella in Brazilian processing plants by real-time quantitative polymerase chain reaction (qPCR).
A total of 139 poultry slaughterhouses samples were collected in order to detect to and quantify Salmonella by qPCR.
Almost all collection points (3/18), except water from pre-chiller tank, carcasses after pre-chiller, and carcasses frozen at -12ºC for 60 days, and 49% (68/139) of samples were positive for Salmonella. Quantification means varied equally among all of the tested sources, and we could not establish any pattern of variation. A large proportion (52.6%) of cloacal swabs was Salmonella-positive. Also, contamination in transport cages was increased after the cleaning process, indicating that the process was ineffective. The overall prevalence in samples obtained during the slaughtering process was 48.9%, and on the whole rinsed carcasses, this proportion was 50%. The detection of Salmonella in frozen carcasses, even after long periods of storage, indicates that the carcasses are a potential source of infection for consumers.
We found that contamination levels remain similar throughout the slaughtering. qPCR proved to be an efficient method for the detection of Salmonella.
沙门氏菌是全球食源性疾病的主要病因。运用定量技术对于评估风险以及确定屠宰过程中每个步骤减少或增加细菌污染的能力而言至关重要。我们旨在通过实时定量聚合酶链反应（qPCR）检测并量化巴西加工厂中沙门氏菌的存在情况。
总共收集了139份家禽屠宰场样本，以便通过qPCR检测和量化沙门氏菌。
几乎所有采集点（18个中的3个），除了预冷槽中的水、预冷后的胴体以及在-12℃冷冻60天的胴体之外，49%（68/139）的样本沙门氏菌呈阳性。所有测试来源的量化均值差异不大，我们无法确定任何变化模式。很大一部分（52.6%）泄殖腔拭子沙门氏菌呈阳性。此外，清洁过程后运输笼中的污染增加，表明该过程无效。屠宰过程中获得的样本总体患病率为48.9%，在整个冲洗后的胴体上，这一比例为50%。即使经过长时间储存，在冷冻胴体中检测到沙门氏菌，表明胴体是消费者潜在的感染源。
我们发现整个屠宰过程中的污染水平保持相似。qPCR被证明是检测沙门氏菌的有效方法。
