Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, RS, Brazil.
J Infect Dev Ctries. 2020 Jan 31;14(1):109-113. doi: 10.3855/jidc.11973.
Campylobacteriosis is considered the most common bacteria-caused human gastroenteritis in the world. Poultry is a major reservoir of Campylobacter. Human infection may occur by consumption of raw and undercooked poultry or by contamination of other foods by these items. The aim of this study was to assess the prevalence of Campylobacter spp. in poultry processing plants with conventional culture method and real-time PCR.
A total of 108 poultry processing plant samples were collected to test with conventional microbiology and qPCR. Sampling included cloacal swabs, swabs of transport crates (before and after the cleaning and disinfection process) and carcasses (after the chiller, cooled at 4°C and frozen at -12°C).
Positivity in cloacal swabs indicated that poultry arrived contaminated at the slaughterhouse. Contamination in transport cages was substantially increased after the cleaning process, indicating that the process was ineffective. The detection of Campylobacter on carcasses was higher than that on cloacal swabs, which could indicate cross-contamination during the slaughtering process. Conventional microbiology and molecular methods revealed a prevalence of 69.4% and 43.5%, respectively. Lower detection by qPCR can be attributed to the high specificity of the kit and to biological components that could inhibit PCR reactions.
Our results indicate that poultry arrive contaminated at the slaughterhouse and that contamination can increase during the slaughtering process due to cross-contamination. The isolation of Campylobacter in cooled and frozen carcasses corroborates the bacterial survival even at temperatures considered limiting to bacterial growth which are routinely used for food preservation.
弯曲菌病被认为是世界上最常见的由细菌引起的人类肠胃炎。家禽是弯曲菌的主要宿主。人类感染可能是通过食用生的和未煮熟的家禽或这些物品污染其他食物引起的。本研究的目的是评估常规培养方法和实时 PCR 检测在禽肉加工厂中弯曲菌属的流行情况。
共采集 108 个禽肉加工厂样本，用常规微生物学和 qPCR 进行检测。采样包括泄殖腔拭子、运输箱拭子（清洁和消毒前后）和胴体（冷却至 4°C 后和冷冻至-12°C）。
泄殖腔拭子的阳性结果表明家禽在进入屠宰场时已受到污染。运输箱在清洁后污染程度显著增加，表明清洁过程无效。在胴体上检测到的弯曲菌比在泄殖腔拭子上检测到的多，这可能表明在屠宰过程中发生了交叉污染。常规微生物学和分子方法的检出率分别为 69.4%和 43.5%。qPCR 检测率较低可能归因于试剂盒的高特异性和可能抑制 PCR 反应的生物成分。
我们的研究结果表明，家禽在进入屠宰场时已受到污染，并且由于交叉污染，在屠宰过程中污染可能会增加。在冷却和冷冻胴体中分离出弯曲菌证实了即使在通常用于食品保存的限制细菌生长的温度下，细菌也能存活。
