Mamuad Lovelia L, Kim Seon Ho, Ku Min Jung, Lee Sang Suk
Ruminant Nutrition and Anaerobe Laboratory, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea.
Livestock Research Institute, Jeonnam Agricultural Research and Extension Services, Gangjin 59213, Korea.
Asian-Australas J Anim Sci. 2020 Jul;33(7):1087-1095. doi: 10.5713/ajas.19.0785. Epub 2020 Jan 13.
The present study aimed to evaluate the effects of γ-aminobutyric acid (GABA)- producing bacteria (GPB) on in vitro rumen fermentation and on the growth performance and meat quality of Hanwoo steers.
The effects of GPB (Lactobacillus brevis YM 3-30)-produced and commercially available GABA were investigated using in vitro rumen fermentation. Using soybean meal as a substrate, either GPB-produced or commercially available GABA were added to the in vitro rumen fermentation bottles, as follows: control, no additive; T1, 2 g/L GPB; T2, 5 g/L GPB; T3, 2 g/L autoclaved GPB; T4, 5 g/L autoclaved GPB; T5, 2 g/L GABA; and T6, 5 g/L GABA. In addition, 27 Hanwoo steers (602.06±10.13 kg) were subjected to a 129-day feeding trial, during which they were fed daily with a commercially available total mixed ration that was supplemented with different amounts of GPB-produced GABA (control, no additive; T1, 2 g/L GPB; T2, 5 g/L GPB). The degree of marbling was assessed using the nine-point beef marbling standard while endotoxin was analyzed using a Chromo-Limulus amebocyte lysate test.
In regard to in vitro rumen fermentation, the addition of GPB-produced GABA failed to significantly affect pH or total gas production but did increase the ammonia nitrogen (NH3-N) concentration (p<0.05) and reduce total biogenic amines (p<0.05). Animals fed the GPB-produced GABA diet exhibited significantly lower levels of blood endotoxins than control animals and yielded comparable average daily gain, feed conversion ratio, and beef marbling scores.
The addition of GPB improved in vitro fermentation by reducing biogenic amine production and by increasing both antioxidant activity and NH3-N production. Moreover, it also reduced the blood endotoxin levels of Hanwoo steers.
本研究旨在评估产γ-氨基丁酸（GABA）细菌（GPB）对体外瘤胃发酵以及韩牛生长性能和肉质的影响。
使用体外瘤胃发酵研究了GPB（短乳杆菌YM 3-30）产生的GABA和市售GABA的效果。以豆粕为底物，将GPB产生的或市售的GABA添加到体外瘤胃发酵瓶中，如下：对照组，不添加；T1，2 g/L GPB；T2，5 g/L GPB；T3，2 g/L经高压灭菌的GPB；T4，5 g/L经高压灭菌的GPB；T5，2 g/L GABA；T6，5 g/L GABA。此外，对27头韩牛（602.06±10.13 kg）进行了为期129天的饲养试验，在此期间，每天给它们饲喂添加不同量GPB产生的GABA的市售全混合日粮（对照组，不添加；T1，2 g/L GPB；T2，5 g/L GPB）。使用九点牛肉大理石花纹标准评估大理石花纹程度，同时使用鲎试剂法分析内毒素。
关于体外瘤胃发酵，添加GPB产生的GABA未能显著影响pH值或总产气量，但确实增加了氨氮（NH3-N）浓度（p<0.05）并降低了总生物胺（p<0.05）。饲喂GPB产生的GABA日粮的动物血液内毒素水平明显低于对照动物，并且平均日增重、饲料转化率和牛肉大理石花纹评分相当。
添加GPB通过减少生物胺产生、增加抗氧化活性和NH3-N产生改善了体外发酵。此外，它还降低了韩牛的血液内毒素水平。
