转录因子 AP2 控制刺胞动物生殖细胞的诱导。
Transcription factor AP2 controls cnidarian germ cell induction.
机构信息
Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA.
出版信息
Science. 2020 Feb 14;367(6479):757-762. doi: 10.1126/science.aay6782.
Abstract
Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in mutants. Forced expression of in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line-sequestering and germ line-nonsequestering animals.
摘要
克隆动物在胚胎发生过程中不会隔离生殖系。相反，它们具有成年干细胞，这些干细胞有助于体组织或配子的形成。在这些动物中，生殖命运是如何被诱导的，以及这个过程是否与两侧胚胎生殖系的诱导有关，目前尚不清楚。我们表明，转录因子 AP2（Tfap2），一种哺乳动物生殖系的调节因子，作用于成年干细胞，称为 i 细胞，使其向克隆腔肠动物 突变体的生殖细胞命运分化，这些突变体缺乏生殖细胞和性腺。野生型细胞的移植挽救了 突变体的性腺发育，但不能诱导生殖细胞的形成。在 i 细胞中强制表达 可将其转化为生殖细胞。因此，Tfap2 是跨隔离生殖系和非隔离生殖系动物的生殖细胞决定的调节因子。
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