Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China.
Sci Rep. 2020 Feb 13;10(1):2509. doi: 10.1038/s41598-020-59439-9.
NJ001 is a monoclonal antibody that can specifically recognize the SP70 antigen on lung adenocarcinoma cells. The goal of this study was to explore its utility in targeted imaging. Subcutaneous xenograft and orthotopic lung tumor implantation BALB/c mouse models were established. Near-infrared fluorescent CF750-labeled NJ001 was injected into two tumor mouse models. Mice that received orthotopic lung tumor implantation were also injected with NJ001-conjugated nanomagnetic beads intravenously, and then underwent micro-CT scanning. Meanwhile, mice with lung tumor were intravenously injected with normal saline and bare nanomagnetic beads as a control. Fluorescence could be monitored in the mice detected by anti-SP70 fluorescence imaging, which was consistent with tumor burden. Signal intensities detected with SP70-targeted micro-CT scans were greater than those in control mice. More importantly, orthotopic tumor lesions could be found on the fourth week with SP70-targeted imaging, which was 2 weeks earlier than detection in the control. Our results suggest that SP70 is a promising target for molecular imaging, and molecularly targeted imaging with an NJ001-labeled probe could be applied for the early detection of lung adenocarcinoma.
NJ001 是一种单克隆抗体，可特异性识别肺腺癌细胞上的 SP70 抗原。本研究旨在探索其在靶向成像中的应用。建立了皮下异种移植和原位肺肿瘤植入 BALB/c 小鼠模型。将近红外荧光 CF750 标记的 NJ001 注入两种肿瘤小鼠模型中。对接受原位肺肿瘤植入的小鼠也经静脉注射 NJ001 偶联的纳米磁珠，然后进行 micro-CT 扫描。同时，对肺肿瘤小鼠静脉注射生理盐水和裸纳米磁珠作为对照。通过抗 SP70 荧光成像可监测到荧光，其与肿瘤负荷一致。用 SP70 靶向 micro-CT 扫描检测到的信号强度大于对照组。更重要的是，SP70 靶向成像可在第 4 周发现原位肿瘤病变，比对照组提前 2 周。我们的结果表明，SP70 是分子成像有前途的靶点，用 NJ001 标记探针进行分子靶向成像可用于肺腺癌的早期检测。
