Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan.
Department of Diagnostic Pathology, Saiseikai-Noe Hospital, Osaka, Japan.
Sci Rep. 2020 Feb 13;10(1):2505. doi: 10.1038/s41598-020-59530-1.
Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family. SLAMF8 suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2. In this study, we found that some anaplastic large cell lymphoma (ALCL) samples were immunohistochemically positive for SLAMF8. However, we found no significant differences between SLAMF8-positive and SLAMF8-negative ALCL samples with respect to age, gender, site, or prognosis. We also identified SLAMF8 expression in ALCL cell lines, Karpas299, and SU-DHL-1. SLAMF8 knockdown decreased the activation of SHP-2 and the growth of these cell lines, and increased the apoptosis of these cell lines. In addition, we observed the interaction between SLAMF8 and SHP-2 in these cell lines using the DuoLink in situ kit. Taken together, these results suggest that SLAMF8 may enhance the growth of ALCL via SHP-2 interaction.
信号淋巴细胞激活分子家族成员 8（SLAMF8）/B 淋巴细胞激活剂巨噬细胞表达/CD353 是 CD2 家族的一员。SLAMF8 抑制巨噬细胞功能，但通过 SHP-2 增强肿瘤性肥大细胞的生长。在这项研究中，我们发现一些间变性大细胞淋巴瘤（ALCL）样本的 SLAMF8 免疫组织化学呈阳性。然而，我们发现 SLAMF8 阳性和 SLAMF8 阴性 ALCL 样本在年龄、性别、部位或预后方面没有显著差异。我们还在 ALCL 细胞系 Karpas299 和 SU-DHL-1 中鉴定出 SLAMF8 的表达。SLAMF8 敲低降低了这些细胞系中 SHP-2 的激活和生长，并增加了这些细胞系的凋亡。此外，我们使用 DuoLink 原位试剂盒观察到这些细胞系中 SLAMF8 和 SHP-2 之间的相互作用。综上所述，这些结果表明 SLAMF8 可能通过与 SHP-2 的相互作用增强 ALCL 的生长。
