Montazeri Mahbobeh, Mirzaee Fatemeh, Daryani Ahmad, Naeimayi Raheleh, Moradi Karimabad Shohre, Khalilzadeh Arjmandi Hadiseh, Esmaealzadeh Niusha, Shahani Somayeh
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Adv Biomed Res. 2020 Jan 21;9:5. doi: 10.4103/abr.abr_206_19. eCollection 2020.
() is a protozoan parasite that infects a wide range of warm-blooded animals and humans. The conventional anti- treatments cause significant toxicity. family contains several medicinal plants with anti-inflammatory, chemopreventive, insecticide, antibacterial, antiviral, and antiparasitic effects. In this study, the hydroalcoholic extract of some species was investigated against .
Seeds of , , , and aerial parts of and were extracted by maceration method using 80% ethanol. Vero cells were treated with different concentrations (5-600 μg/mL) of the extracts and pyrimethamine (as positive control), and the cellular viability was verified. Next, Vero cells were infected by tachyzoites (RH strain), and the viability of the infected cells was measured by a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
The 50% inhibitory concentration values were 5.1, 14.67, 32.49, 37.31, 71.35, and 2.63 μg/mL, and the selectivity indices were 8.06, 2.59, 0.74, 0.78, 0.65 ( < 0.05 compared with positive control), and 3.03 for , , , , , and pyrimethamine, respectively.
The results of this study demonstrated that the hydroalcoholic extracts of and have the promising anti- activity by growth inhibition of tachyzoites in infected cells.
（）是一种原生动物寄生虫，可感染多种温血动物和人类。传统的抗（寄生虫）治疗会产生显著的毒性。（某）科包含几种具有抗炎、化学预防、杀虫、抗菌、抗病毒和抗寄生虫作用的药用植物。在本研究中，对某些（该科）物种的水醇提取物进行了抗（该寄生虫）研究。
（该科几种植物的名称，此处未给出具体中文译名）的种子以及（另外几种植物的名称，此处未给出具体中文译名）的地上部分通过使用80%乙醇的浸渍法进行提取。用不同浓度（5 - 600μg/mL）的提取物和乙胺嘧啶（作为阳性对照）处理Vero细胞，并验证细胞活力。接下来，用（该寄生虫的）速殖子（RH株）感染Vero细胞，并通过比色法3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐法测定感染细胞的活力。
50%抑制浓度值分别为5.1、14.67、32.49、37.31、71.35和2.63μg/mL，选择性指数分别为8.06、2.59、0.74、0.78、0.65（与阳性对照相比，P < 0.05）和3.03，分别对应（几种植物提取物及乙胺嘧啶）。
本研究结果表明，（某两种植物名称，此处未给出具体中文译名）的水醇提取物通过抑制感染细胞中（该寄生虫）速殖子的生长具有有前景的抗（该寄生虫）活性。
