Ebrahimzadeh Mohammad Ali, Taheri Mohammad Mohammad, Ahmadpour Ehsan, Montazeri Mahbobeh, Sarvi Shahabeddin, Akbari Mohammad, Daryani Ahmad
Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences Sari, Iran.
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Pharmacopuncture. 2017 Sep;20(3):220-226. doi: 10.3831/KPI.2017.20.026. Epub 2017 Sep 30.
The currently available agents for use against toxoplasmosis have serious limitations. Thus, the aim of the present study was to investigate the anti- () activities of methanol extracts of () (leaves and fruits), () (fruits), and () (leaves) and .
Vero cells were treated with different concentrations (from 0 to 400 μg/mL) of the above extracts or with pyrimethamine at a dose of 50 mg/mL (positive control). Then, the viabilities of the -infected cells were measured by using colorimetric MTT (3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide) assays. In addition, the survival rates of mice acutely infected with 2 × 10 RH strain tachyzoites of were examined after intraperitoneal injection of the extracts at doses of 100 and 200 mg/kg/day for 5 days.
In the anti- assay, the IC values were 12.77, 180.2, 74.73, 213.2 and 163.8 μg/mL, and the selectivity indices were 6.05, 1.31, 0.35, 0.69 and 1.30 for the (leaves and fruits), , and extracts and pyrimethamine, respectively. Moreover, the mice treated with (leaves and fruits) achieved better results in terms of survival than the others ( < 0.05).
The results of the current study indicate that methanol extract of has significant anti- activity. Further study should be conducted to investigate the potential bioactivity of this extract through bioactivity-guided fractionation.
目前可用的抗弓形虫病药物存在严重局限性。因此，本研究的目的是调查[植物名称1]（叶和果实）、[植物名称2]（果实）和[植物名称3]（叶）的甲醇提取物以及[提取物名称]的抗（弓形虫）活性。
用上述不同浓度（0至400μg/mL）的提取物或50mg/mL剂量的乙胺嘧啶（阳性对照）处理Vero细胞。然后，使用比色MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）测定法测量被弓形虫感染细胞的活力。此外，在以100和200mg/kg/天的剂量腹腔注射提取物5天后，检查急性感染2×10[弓形虫株名称]速殖子的小鼠的存活率。
在抗弓形虫试验中，[植物名称1]（叶和果实）、[植物名称2]、[植物名称3]提取物以及乙胺嘧啶的IC值分别为12.77、180.2、74.73、213.2和163.8μg/mL，选择性指数分别为6.05、1.31、0.35、0.69和1.30。此外，用[植物名称1]（叶和果实）处理的小鼠在存活率方面比其他组取得了更好的结果（P<0.05）。
本研究结果表明[植物名称1]的甲醇提取物具有显著的抗弓形虫活性。应通过生物活性导向分级分离进一步研究该提取物的潜在生物活性。
