Kim Yunjeong, Jeon Ji-Young, Han Song-Hee, Ha Na, Jang Kyungho, Kim Min-Gul
Center for Clinical Pharmacology and Biomedical Research Institute, Chonbuk National University Hospital, Jeonju 54907, Republic of Korea.
Department of Pharmacology, School of Medicine, Chonbuk National University, Jeonju 54907, Republic of Korea.
Transl Clin Pharmacol. 2018 Mar;26(1):32-38. doi: 10.12793/tcp.2018.26.1.32. Epub 2018 Mar 16.
Volumetric absorptive microsampling (VAMS) is a novel sampling technique that allows for the collection of an accurate volume of blood by dipping a microsampler tip. The purpose of this study is to compare the requirement of a stabilizing reagent for the conventional venous blood sampling method versus VAMS in the analytical measurement of the concentration of acetylsalicylic acid. A high-performance liquid chromatography with mass spectrometry (LC-MS/MS) method was developed and validated for the accurate determination of acetylsalicylic acid in human blood. The blood samples spiked with acetylsalicylic acid with and without stabilizing reagent were absorbed into VAMS tips. In the whole blood sample, the same concentration was shown regardless of the addition of the stabilizing reagent, but the concentration decreased when the stabilizing reagent was not added to the VAMS sample. To apply the VAMS technology as a new blood sampling method, stabilizing reagents should be added before the analysis of acetylsalicylic acid concentration.
体积吸收微采样（VAMS）是一种新型采样技术，通过将微采样器尖端浸入可采集准确体积的血液。本研究的目的是比较传统静脉采血法与VAMS在乙酰水杨酸浓度分析测量中对稳定试剂的需求。开发并验证了一种高效液相色谱-质谱联用（LC-MS/MS）方法用于准确测定人血中的乙酰水杨酸。将添加和未添加稳定试剂的含乙酰水杨酸的血样吸入VAMS尖端。在全血样本中，无论是否添加稳定试剂，浓度均相同，但在VAMS样本中未添加稳定试剂时浓度会降低。为将VAMS技术作为一种新的采血方法应用，在分析乙酰水杨酸浓度之前应添加稳定试剂。
