不依赖血细胞比容的回收率是使用体积吸收微采样装置Mitra™进行生物分析的关键。
Hematocrit-independent recovery is a key for bioanalysis using volumetric absorptive microsampling devices, Mitra™.
作者信息
Mano Yuji, Kita Kenji, Kusano Kazutomi
机构信息
Drug Metabolism & Pharmacokinetics, Biopharmaceutical Assessment Core Function Unit, Eisai Co., Ltd. 1-3, 5-chome, Tokodai, Tsukuba-shi, Ibaraki 300-2635, Japan.
Bioanalysis Unit, Tsukuba R&D supporting division, Sunplanet Co., Ltd. 1-3, 5-chome, Tokodai, Tsukuba-shi, Ibaraki 300-2635, Japan.
出版信息
Bioanalysis. 2015;7(15):1821-9. doi: 10.4155/bio.15.111.
BACKGROUND
A novel microsampling device, Mitra(TM), was evaluated for bioanalysis of E6005 and its O-desmethylated metabolite in human whole blood using an UPLC-MS.
RESULTS
A constant volume of blood samples was absorbed onto the tip of Mitra, the analytes were extracted by various solvents and then detected by UPLC-MS. Recovery of the analytes was high in acetonitrile-water (1:1, v/v) but was dependent on hematocrit (Hct) without sonication process, which led to biased accuracy at low and high Hcts. Inclusion of sonication process in extraction improved recovery at high Hct to yield acceptable accuracy across Hcts.
CONCLUSION
Optimization of extraction process to achieve high recovery regardless of Hct is critical in accurate bioanalysis via Mitra.
背景
使用超高效液相色谱-质谱联用仪（UPLC-MS）对一种新型微量采样装置Mitra(TM)进行了评估，用于人全血中E6005及其O-去甲基代谢物的生物分析。
结果
固定体积的血样被吸附到Mitra的尖端，分析物用各种溶剂萃取，然后通过UPLC-MS进行检测。在乙腈-水（1:1，v/v）中分析物的回收率很高，但在没有超声处理的情况下，回收率取决于血细胞比容（Hct），这导致在低血细胞比容和高血细胞比容时准确度出现偏差。在萃取过程中加入超声处理可提高高血细胞比容时的回收率，从而在不同血细胞比容下获得可接受的准确度。
结论
通过Mitra进行准确的生物分析时，优化萃取过程以实现无论血细胞比容如何都具有高回收率至关重要。
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