一种新型γ-拟病毒感染真菌稻曲病菌的分子特征。
Molecular characterization of a novel gammapartitivirus infecting the fungus Nigrospora oryzae.
机构信息
Dali Tobacco Company of Yunnan Province, Dali City, Yunnan Province, 671000, P.R. China.
Qujing Tobacco Company of Yunnan Province, Qujing Yunnan Province, 655000, P.R. China.
出版信息
Arch Virol. 2024 Aug 26;169(9):188. doi: 10.1007/s00705-024-06111-6.
Here, we identified a new mycovirus infecting the phytopathogenic fungus Nigrospora oryzae, which we have designated "Nigrospora oryzae partitivirus 2" (NoPV2). The genome of NoPV2 consists of two dsRNA segments (dsRNA 1 and dsRNA 2), measuring 1771 and 1440 bp in length, respectively. dsRNA 1 and dsRNA 2 each contain a single open reading frame (ORF) that encodes the RNA-dependent RNA polymerase (RdRp) and capsid protein (CP), respectively. A BLASTp search showed that the RdRp of NoPV2 had significant sequence similarity to the RdRps of other partitiviruses, including Nigrospora sphaerica partitivirus 1 (75.61% identity) and Magnaporthe oryzae partitivirus 1 (67.53% identity). Phylogenetic analysis revealed that NoPV2 is a new member of the genus Gammapartitivirus in the family Partitiviridae. This study provides important information for understanding the diversity of mycoviruses in N. oryzae.
在这里，我们鉴定了一种新的感染植物病原菌稻曲病菌的真菌病毒，我们将其命名为“稻曲病菌二分体病毒 2 号”（NoPV2）。NoPV2 的基因组由两个 dsRNA 片段（dsRNA1 和 dsRNA2）组成，分别长 1771 和 1440bp。dsRNA1 和 dsRNA2 各自包含一个单一的开放阅读框（ORF），分别编码 RNA 依赖的 RNA 聚合酶（RdRp）和衣壳蛋白（CP）。BLASTp 搜索表明，NoPV2 的 RdRp 与其他二分体病毒的 RdRps 具有显著的序列相似性，包括黑曲霉二分体病毒 1（75.61%的同一性）和稻瘟病菌二分体病毒 1（67.53%的同一性）。系统进化分析表明，NoPV2 是二分体病毒科二分体病毒属的一个新成员。本研究为了解稻曲病菌中真菌病毒的多样性提供了重要信息。
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