Dali Tobacco Company of Yunnan Province, Dali City, Yunnan Province, 671000, P.R. China.
Qujing Tobacco Company of Yunnan Province, Qujing Yunnan Province, 655000, P.R. China.
Arch Virol. 2024 Aug 26;169(9):188. doi: 10.1007/s00705-024-06111-6.
Here, we identified a new mycovirus infecting the phytopathogenic fungus Nigrospora oryzae, which we have designated "Nigrospora oryzae partitivirus 2" (NoPV2). The genome of NoPV2 consists of two dsRNA segments (dsRNA 1 and dsRNA 2), measuring 1771 and 1440 bp in length, respectively. dsRNA 1 and dsRNA 2 each contain a single open reading frame (ORF) that encodes the RNA-dependent RNA polymerase (RdRp) and capsid protein (CP), respectively. A BLASTp search showed that the RdRp of NoPV2 had significant sequence similarity to the RdRps of other partitiviruses, including Nigrospora sphaerica partitivirus 1 (75.61% identity) and Magnaporthe oryzae partitivirus 1 (67.53% identity). Phylogenetic analysis revealed that NoPV2 is a new member of the genus Gammapartitivirus in the family Partitiviridae. This study provides important information for understanding the diversity of mycoviruses in N. oryzae.
在这里，我们鉴定了一种新的感染植物病原菌稻曲病菌的真菌病毒，我们将其命名为“稻曲病菌二分体病毒 2 号”（NoPV2）。NoPV2 的基因组由两个 dsRNA 片段（dsRNA1 和 dsRNA2）组成，分别长 1771 和 1440bp。dsRNA1 和 dsRNA2 各自包含一个单一的开放阅读框（ORF），分别编码 RNA 依赖的 RNA 聚合酶（RdRp）和衣壳蛋白（CP）。BLASTp 搜索表明，NoPV2 的 RdRp 与其他二分体病毒的 RdRps 具有显著的序列相似性，包括黑曲霉二分体病毒 1（75.61%的同一性）和稻瘟病菌二分体病毒 1（67.53%的同一性）。系统进化分析表明，NoPV2 是二分体病毒科二分体病毒属的一个新成员。本研究为了解稻曲病菌中真菌病毒的多样性提供了重要信息。
