Snyder J M, Kwun J E, O'Brien J A, Rosenfeld C R, Odom M J
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235.
Pediatr Res. 1988 Dec;24(6):728-34. doi: 10.1203/00006450-198812000-00016.
A specific, enzyme-linked immunoabsorbent assay was used to determine the concentration of the 35,000 mol wt surfactant apoprotein (SP-A) in samples of amniotic fluid obtained from nondiabetic (n = 358) and diabetic (n = 29) women. The enzyme-linked immunoabsorbent assay was performed with rabbit antibodies directed against SP-A present in lavage fluid from a patient with alveolar proteinosis. Amniotic fluid SP-A concentrations increased as a function of gestational age, from less than 3 micrograms/ml at 30-31 wk to 24 micrograms/ml at 40-41 wk, and were positively correlated with the lecithin to sphingomyelin ratio (p less than 0.01). SP-A concentrations also increased as a function of gestational age in shake test positive samples (p less than 0.05), but were unchanged in shake test-negative samples. There was no difference in the surfactant apoprotein concentration of male compared with female fetuses at any gestational age. In amniotic fluid obtained from 20 diabetic women, SP-A levels were significantly less than in nondiabetic pregnancies that were matched for gestational age and sex of the fetus (p less than 0.05). The SP-A concentrations in amniotic fluids obtained from nine women who were diabetic and hypertensive and from 10 hypertensive women were not different from matched controls. The relationships described above were valid whether the SP-A concentration was expressed per mg protein or per ml amniotic fluid. These data are suggestive that the concentration of amniotic fluid SP-A is decreased in diabetic pregnancies.
采用一种特异性的酶联免疫吸附测定法，来测定从非糖尿病孕妇（n = 358）和糖尿病孕妇（n = 29）获取的羊水样本中分子量为35,000道尔顿的表面活性物质载脂蛋白（SP-A）的浓度。酶联免疫吸附测定使用的是兔抗体，该抗体针对的是肺泡蛋白沉积症患者灌洗液中存在的SP-A。羊水SP-A浓度随孕周增加而升高，从30 - 31周时的低于3微克/毫升升至40 - 41周时的24微克/毫升，且与卵磷脂/鞘磷脂比值呈正相关（p < 0.01）。在振荡试验阳性的样本中，SP-A浓度也随孕周增加而升高（p < 0.05），但在振荡试验阴性的样本中无变化。在任何孕周，男胎与女胎的表面活性物质载脂蛋白浓度均无差异。在从20名糖尿病孕妇获取的羊水中，SP-A水平显著低于与之孕周和胎儿性别匹配的非糖尿病孕妇（p < 0.05）。从9名患有糖尿病和高血压的女性以及10名高血压女性获取的羊水样本中的SP-A浓度与匹配的对照组无差异。无论SP-A浓度是以每毫克蛋白还是每毫升羊水来表示，上述关系均成立。这些数据提示糖尿病妊娠时羊水SP-A浓度降低。
