Paciga J E, Shelley S A, Paterson J E, Knuppel R A, Scerbo J C, Balis J U
Department of Pathology, University of South Florida, College of Medicine, Tampa 33612.
Ann Clin Lab Sci. 1988 Mar-Apr;18(2):141-7.
Dot immunobinding assays for the quantitation of two classes of proteins associated with lung surfactant phospholipids in human amniotic fluid are described. With the use of these assays it was determined that the two classes of surfactant proteins accumulate in the amniotic fluid at the same rate. The concentrations of disaturated phosphatidylcholine and the surfactant-associated proteins are less closely correlated. Centrifugation of amniotic fluids either before or after freezing resulted in a loss ranging from 10 to 35 percent of both surfactant disaturated phosphatidylcholine and proteins depending on the relative centrifugal force used. Preterm amniotic fluids contained significantly less of both surfactant-associated proteins, as well as disaturated phosphatidylcholine, than did term amniotic fluids which suggests that the use of these specific protein markers may enhance the assessment of fetal lung maturity.
本文描述了用于定量分析人羊水与肺表面活性物质磷脂相关的两类蛋白质的斑点免疫结合测定法。使用这些测定法确定，这两类表面活性物质蛋白质在羊水中以相同速率积累。二饱和磷脂酰胆碱与表面活性物质相关蛋白质的浓度相关性较弱。冷冻前后对羊水进行离心，根据所用相对离心力的不同，表面活性物质二饱和磷脂酰胆碱和蛋白质的损失率在10%至35%之间。早产羊水所含的表面活性物质相关蛋白质以及二饱和磷脂酰胆碱均明显少于足月羊水，这表明使用这些特定的蛋白质标志物可能会增强对胎儿肺成熟度的评估。
