Clinical Microbiology Laboratories, Polyclinic of Tor Vergata, Rome, Italy.
Department of Experimental Medicine & Biochemical Sciences, University of Rome "Tor Vergata", Rome, Italy.
Future Microbiol. 2020 Feb;15:203-212. doi: 10.2217/fmb-2019-0074. Epub 2020 Feb 14.
Carbapenemase-resistant represents a major concern in hospital setting. The evolutionary history of carbapenem-resistant strains was analyzed by core genome multilocus sequence typing and Bayesian phylogenesis by whole genomes sequencing. A great increase carbapenem-resistant causing blood stream infection was observed in the years 2015-2016. At multilocus sequence typing (MLST), they were prevalently ST512 and ST101. ST512 were core genome (cg)MLST 53, while ST101 mainly cgMLST453. The minimum-spanning tree, based on cgMLST, showed strains clustering based on the different STs. By Bayesian phylogenetic analysis, maximum clade credibility tree showed that strains were introduced in the year 2005 with the most probable location in the ICU ward. Two outbreaks by ST101 and ST512 strains with Tower T8 as the probable location were evidenced. Molecular epidemiology is a powerful tool to track the way of transmission of resistant bacteria within the hospital setting.
耐碳青霉烯肠杆菌代表了医院环境中的一个主要关注点。通过核心基因组多位点序列分型和全基因组测序的贝叶斯系统发育分析，研究了耐碳青霉烯肠杆菌菌株的进化历史。在 2015 年至 2016 年期间，耐碳青霉烯肠杆菌引起的血流感染显著增加。在多位点序列分型（MLST）中，它们主要是 ST512 和 ST101。ST512 是核心基因组（cg）MLST 53，而 ST101 主要是 cgMLST453。基于 cgMLST 的最小生成树显示，菌株根据不同的 ST 聚类。通过贝叶斯系统发育分析，最大分支置信度树表明，这些菌株于 2005 年引入，最有可能的位置是 ICU 病房。有证据表明，ST101 和 ST512 菌株爆发了两次，Tower T8 可能是传染源。分子流行病学是一种强大的工具，可以追踪医院环境中耐药菌的传播途径。
