Department of Maternal and Child Health and MOE (Ministry of Education) Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430015, China.
J Affect Disord. 2020 Mar 1;264:304-309. doi: 10.1016/j.jad.2020.01.017. Epub 2020 Jan 7.
Brain-derived neurotrophic factor (BDNF) is vital for neuronal survival and growth, regulation of synaptic plasticity, and cognitive function. Previous studies examined the role of BDNF in susceptibility to attention deficit hyperactivity disorder (ADHD). The current study examined the association between BDNF gene polymorphisms and ADHD in Chinese children.
Participants were 195 medication-naive ADHD children and 263 unaffected healthy controls. ADHD symptoms were diagnosed using the Vanderbilt ADHD Diagnostic Parental Rating Scale according to the Diagnostic and Statistical Manual of Mental Disorders-5. Five BDNF single nucleotide polymorphisms were detected using improved multiplex ligation detection reaction. Alleles and genotype frequency were examined using Chi-square tests. Correlations were examined using multivariate logistic regression analysis in the ADHD and control groups.
The results revealed that rs12291186 with one or both mutated allele(s) was significantly associated with reduced likelihood of ADHD (OR = 0.13, 95% CI: 0.02-0.77) and ADHD-Combined (OR = 0.10, 95% CI: 0.01-0.85). Children with genotype AA or CA in rs10835210 exhibited increased risk of ADHD (OR = 3.29, 95% CI: 1.03-10.55) and ADHD-Combined (OR = 4.45, 95% CI: 1.10-17.96) compared with genotype CC children. No significant associations were found between rs6265, rs7103411, rs7103873 polymorphisms and ADHD.
Participants were recruited from urban areas. We were unable to examine all potential confounding factors.
BDNF gene polymorphisms of rs12291186 and rs10835210 were related to the occurrence of ADHD. These findings provide new insight on mechanisms underlying BDNF gene in ADHD.
脑源性神经营养因子（BDNF）对神经元的存活和生长、突触可塑性的调节以及认知功能至关重要。先前的研究探讨了 BDNF 在易患注意缺陷多动障碍（ADHD）中的作用。本研究探讨了 BDNF 基因多态性与中国儿童 ADHD 的关系。
参与者为 195 名未经药物治疗的 ADHD 儿童和 263 名未受影响的健康对照。根据《精神障碍诊断与统计手册-5》，采用范德比尔特 ADHD 诊断父母评定量表诊断 ADHD 症状。使用改良多重连接检测反应检测 5 个 BDNF 单核苷酸多态性。采用卡方检验检测等位基因和基因型频率。在 ADHD 组和对照组中，采用多元逻辑回归分析进行相关性分析。
结果显示，rs12291186 携带一个或两个突变等位基因与 ADHD（OR=0.13，95%CI：0.02-0.77）和 ADHD-混合型（OR=0.10，95%CI：0.01-0.85）的可能性降低显著相关。rs10835210 中基因型为 AA 或 CA 的儿童患 ADHD（OR=3.29，95%CI：1.03-10.55）和 ADHD-混合型（OR=4.45，95%CI：1.10-17.96）的风险高于 CC 基因型儿童。rs6265、rs7103411、rs7103873 多态性与 ADHD 之间无显著相关性。
参与者均来自城区。我们无法检测所有潜在的混杂因素。
rs12291186 和 rs10835210 的 BDNF 基因多态性与 ADHD 的发生有关。这些发现为 BDNF 基因在 ADHD 中的作用机制提供了新的见解。
