Kwon Ho Jang, Ha Mina, Jin Han Jun, Hyun Jung Keun, Shim Se Hoon, Paik Ki Chung, Park Woo Sung, Lim Myung Ho
1 Environmental Health Center, Dankook Medical Hospital , Cheonan, South Korea .
2 Department of Preventive Medicine, College of Medicine, Dankook University , Cheonan, South Korea .
Genet Test Mol Biomarkers. 2015 Jul;19(7):366-71. doi: 10.1089/gtmb.2015.0029. Epub 2015 May 7.
Attention deficit hyperactivity disorder (ADHD) is a common childhood neuropsychiatric disorder characterized by behavioral problems such as attention deficit, hyperactivity, and impulsivity. The brain-derived neurotrophic factor (BDNF) is the most abundant neurotrophin in the brain.
The aim of the present study was to investigate the association between the genotype and alleles for the BDNF gene in Korean children with ADHD.
The sample consisted of 180 ADHD children and 159 control children. We diagnosed ADHD according to the DSM-IV. ADHD symptoms were evaluated with Conners' Parent Rating Scales and Dupaul Parent ADHD Rating Scales. Blood samples were taken from the 339 subjects, DNA was extracted from blood lymphocytes, and polymerase chain reaction was performed for BDNF rs6265, rs11030101, rs10835210, rs7103873, and rs2030324 polymorphisms. Alleles and genotype frequencies were compared using the Chi-square test. We compared the allele and genotype frequencies of the BDNF gene polymorphism in the ADHD and control groups.
This study showed that there was a significant correlation among the allele frequencies of the rs11030101 and rs10835210 single nucleotide polymorphisms (SNPs) (odds ratio=0.61, 95% confidence interval=0.39-0.96, p=0.034), but the final conclusions are not definite. Follow-up studies with larger patient or pure subgroups are expected. These results suggest that the BDNF allelic structure may impact ADHD symptoms.
注意力缺陷多动障碍（ADHD）是一种常见的儿童神经精神疾病，其特征为注意力不集中、多动和冲动等行为问题。脑源性神经营养因子（BDNF）是大脑中含量最丰富的神经营养因子。
本研究旨在调查韩国ADHD儿童中BDNF基因的基因型和等位基因之间的关联。
样本包括180名ADHD儿童和159名对照儿童。我们根据《精神疾病诊断与统计手册》第四版（DSM-IV）诊断ADHD。使用康纳斯父母评定量表和杜保罗父母ADHD评定量表评估ADHD症状。从339名受试者采集血样，从血液淋巴细胞中提取DNA，并对BDNF rs6265、rs11030101、rs10835210、rs7103873和rs2030324多态性进行聚合酶链反应。使用卡方检验比较等位基因和基因型频率。我们比较了ADHD组和对照组中BDNF基因多态性的等位基因和基因型频率。
本研究表明，rs11030101和rs10835210单核苷酸多态性（SNP）的等位基因频率之间存在显著相关性（优势比=0.61，95%置信区间=0.39 - 0.96，p = 0.034），但最终结论尚不明确。预计将对更大的患者群体或纯亚组进行后续研究。这些结果表明BDNF等位基因结构可能影响ADHD症状。
