Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
Department of Obstetrics, The Second Hospital of Hebei Medical University, No. 215 Heping Xi Road, Shijiazhuang, 050000, Hebei, China.
Placenta. 2020 Mar;92:37-43. doi: 10.1016/j.placenta.2020.02.003. Epub 2020 Feb 5.
The biological functions of placental trophoblast cells have been reported to be critical in preeclampsia (PE) and its complications. Here, we aimed to investigate the role and underlying mechanism of soluble fms-like tyrsine kinase-1 (sFlt-1) and miR-139-5p in severe preeclampsia (sPE) by culturing the trophoblast cells from patients.
ELISA and qRT-PCR were used to measure the expression of sFlt-1 and miR-139-5p. The direct interaction between sFlt-1 and miR-139-5p was determined by luciferase reporter assay. Cell proliferation and invasion were evaluated by CCK-8 analysis and transwell assay.
Our results showed that miR-139-5p was downregulated in sPE patients and was negatively correlated with the expression of sFlt-1. Further, sFlt-1 was a direct target of miR-139-5p, which monitored the expression of sFlt-1. Besides, miR-139-5p promoted the proliferation and invasion of trophoblast cells derived from sPE patients. Overexpression of sFlt-1 attenuated the effects of miR-139-5p on cell proliferation and invasion of trophoblast cells from sPE patients.
Our research proposes a novel mechanism where the role of miR-139-5p is dependent on sFlt-1. Our data demonstrated that miR-139-5p promoted the proliferation and invasion of trophoblast cells by directly targeting sFlt-1 in PE.
胎盘滋养层细胞的生物学功能已被报道在子痫前期（PE）及其并发症中起关键作用。在这里，我们旨在通过培养患者的滋养层细胞来研究可溶性 fms 样酪氨酸激酶-1（sFlt-1）和 miR-139-5p 在重度子痫前期（sPE）中的作用及其潜在机制。
采用 ELISA 和 qRT-PCR 检测 sFlt-1 和 miR-139-5p 的表达。通过荧光素酶报告基因实验测定 sFlt-1 和 miR-139-5p 之间的直接相互作用。通过 CCK-8 分析和 Transwell 测定评估细胞增殖和侵袭。
我们的结果表明，miR-139-5p 在 sPE 患者中下调，与 sFlt-1 的表达呈负相关。此外，sFlt-1 是 miR-139-5p 的直接靶标，可监测 sFlt-1 的表达。此外，miR-139-5p 促进了来自 sPE 患者的滋养层细胞的增殖和侵袭。sFlt-1 的过表达减弱了 miR-139-5p 对 sPE 患者滋养层细胞增殖和侵袭的影响。
我们的研究提出了一种新的机制，其中 miR-139-5p 的作用依赖于 sFlt-1。我们的数据表明，miR-139-5p 通过直接靶向 sFlt-1 促进 PE 中滋养层细胞的增殖和侵袭。
