Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Med J. 2021 Aug;62(8):726-733. doi: 10.3349/ymj.2021.62.8.726.
This study aimed to elucidate whether microRNA-139-5p is involved in the pathogenesis of uterine leiomyoma.
Human leiomyoma and matched human smooth muscle samples were obtained from 10 women who underwent hysterectomy for uterine leiomyoma. MicroRNA (miRNA) expression was analyzed by quantitative real-time polymerase chain reaction. To assess the effects of miR-139-5p on cultured leiomyoma cells, cell migration, collagen gel contraction, wound healing, and the expression levels of hallmark proteins were evaluated in cells transfected with a miR-139-5p mimic.
The expression of miR-139-5p was significantly lower in leiomyoma tissues than in matched smooth muscle tissues. Restored miR-139-5p expression in miR-139-5p mimic-transfected human leiomyoma cells resulted in decreased contractility of the ECM and cell migration. In addition, upregulation of miR-139-5p decreased the protein expression of collagen type 1 and phosphorylated p38 MAPK.
Expression of miR-139-5p is downregulated in leiomyoma cells and modulation of miR-139-5p may be involved inthe pathogenesis of leiomyomas through the regulation of collagen type 1 and phosphorylated p38 MAPK. Therefore, miR-139-5p is a potential therapeutic target for leiomyoma.
本研究旨在阐明 microRNA-139-5p 是否参与子宫肌瘤的发病机制。
从 10 名因子宫肌瘤接受子宫切除术的女性中获得人子宫肌瘤和匹配的人平滑肌样本。通过定量实时聚合酶链反应分析 microRNA (miRNA) 表达。为了评估 miR-139-5p 对培养的子宫肌瘤细胞的影响，用 miR-139-5p 模拟物转染的细胞中评估细胞迁移、胶原凝胶收缩、伤口愈合和标志性蛋白的表达水平。
miR-139-5p 在子宫肌瘤组织中的表达明显低于匹配的平滑肌组织。在 miR-139-5p 模拟物转染的人子宫肌瘤细胞中恢复 miR-139-5p 表达导致 ECM 的收缩性和细胞迁移减少。此外，上调 miR-139-5p 降低了胶原蛋白 1 和磷酸化 p38 MAPK 的蛋白表达。
miR-139-5p 在子宫肌瘤细胞中表达下调，miR-139-5p 的调节可能通过调节胶原蛋白 1 和磷酸化 p38 MAPK 参与子宫肌瘤的发病机制。因此，miR-139-5p 是子宫肌瘤的一个潜在治疗靶点。
