College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, People's Republic of China.
South China Agricultural University Hospital, Guangzhou 510642, Guangdong, People's Republic of China.
Gen Comp Endocrinol. 2020 Jun 1;292:113435. doi: 10.1016/j.ygcen.2020.113435. Epub 2020 Feb 11.
In orange-spotted grouper, androgen can promote the development of testis and spermatogenesis, but the effect of androgen on testis development is unclear. Forkhead box L 3 (Foxl3) is important in the development of fish testis. Rec8 and fbxo47 are involved in meiosis, which impacts spermatogenesis. The present study investigated the plausible role of testis development through the Foxl3 transcriptional regulation of rec8 and fbxo47. The results of tissue distribution showed that rec8 and fbxo47 are highly expressed in gonad. In addition, the highest expression of foxl3, rec8, and fbxo47 was in the testis and intersex compared with the other stages of gonadal development, suggesting that foxl3, rec8, and fbxo47 are important in testis development. In addition, by using dual-luciferase assays, we found that the androgen can increase foxl3 promoter activity and Foxl3 can upregulate rec8 and fbxo47 promoter activity. Furthermore, the addition of β-testosterone significantly increased foxl3, rec8, and fbxo47 promoter activity. Together, these results suggest that foxl3 plays a decisive role in testis development by regulating the expression of rec8 or fbxo47 and imply that AR-foxl3-rec8/fbxo47 affects the testis development pathway.
在橙色斑点石斑鱼中，雄激素可以促进睾丸和精子发生的发育，但雄激素对睾丸发育的影响尚不清楚。叉头框 L3（Foxl3）在鱼类睾丸发育中很重要。Rec8 和 fbxo47 参与减数分裂，这会影响精子发生。本研究通过 Foxl3 对 rec8 和 fbxo47 的转录调控来探讨睾丸发育的可能作用。组织分布的结果表明，rec8 和 fbxo47 在性腺中高度表达。此外，foxl3、rec8 和 fbxo47 的表达量在睾丸和两性中最高，与其他性腺发育阶段相比，这表明 foxl3、rec8 和 fbxo47 在睾丸发育中很重要。此外，通过双荧光素酶测定，我们发现雄激素可以增加 foxl3 启动子活性，Foxl3 可以上调 rec8 和 fbxo47 启动子活性。此外，β-睾酮的添加显著增加了 foxl3、rec8 和 fbxo47 启动子活性。综上所述，这些结果表明，foxl3 通过调节 rec8 或 fbxo47 的表达在睾丸发育中起决定性作用，并暗示 AR-foxl3-rec8/fbxo47 影响睾丸发育途径。
