Duan Xuzhuo, Jia Xianze, Liang Kaishan, Huang Fengqi, Shan Jinhong, Chen Huitao, Ruan Xinhe, Li Lihua, Zhao Huihong, Wang Qing
College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Mar Biotechnol (NY). 2022 Apr;24(2):345-353. doi: 10.1007/s10126-022-10111-5. Epub 2022 Mar 18.
In fish, the maturity of gonads plays an important role in the development and reproduction of the population, and it also dictates the success of captive breeding. Therefore, finding ways to promote gonadal maturation is an important goal in aquaculture. In this study, we injected recombinant dmrt1 and rec8 overexpression plasmids packaged in liposomes into the immature testis of red-spotted grouper (Epinephelus akaara) and measured the expression of Dmrt1 and Rec8 protein in vivo. Gonadosomatic index (GSI) and gonadal histology analyses showed that the testis developed from the immature to the mature state within 7 days after plasmid injection. Additionally, the spermatozoa concentration and motility in plasmid-injected fish was the same as that of naturally mature fish. These results provided evidence that delivery of dmrt1 and rec8 expression plasmids into the testis via injection induced testis maturation in vivo.
在鱼类中，性腺成熟在种群的发育和繁殖中起着重要作用，它还决定了圈养繁殖的成功与否。因此，寻找促进性腺成熟的方法是水产养殖中的一个重要目标。在本研究中，我们将包裹在脂质体中的重组dmrt1和rec8过表达质粒注射到红斑石斑鱼（Epinephelus akaara）未成熟的睾丸中，并在体内测量Dmrt1和Rec8蛋白的表达。性腺指数（GSI）和性腺组织学分析表明，注射质粒后7天内，睾丸从未成熟状态发育到成熟状态。此外，注射质粒的鱼的精子浓度和活力与自然成熟的鱼相同。这些结果证明，通过注射将dmrt1和rec8表达质粒递送至睾丸可在体内诱导睾丸成熟。
