Department of Comparative Biomedical Sciences, LSU School of Veterinary Medicine, Baton Rouge, Louisiana, USA.
Neuroreport. 2020 Mar 4;31(4):332-337. doi: 10.1097/WNR.0000000000001408.
Propagating neural waves in the cerebral cortex influence the integration of incoming sensory information with ongoing cortical activity. However, the neural circuit elements that support these cortical waves remain to be fully defined. Here, a novel tangential slice preparation was developed that exhibited propagating wave activity across the dorsal cortical sheet, as assessed using autofluorescence imaging following focal electrical stimulation. Analysis of functional connectivity in the slice preparation with laser-scanning photostimulation via glutamate uncaging revealed a lack of short-latency, presumed monosynaptic, long-range connections (>300 μm) in the slice preparation. These results establish a novel slice preparation for assessing cortical dynamics and support the proposition that interactions among local cortical elements are sufficient to enable widespread propagating wave activity.
在大脑皮层中传播的神经波影响传入感觉信息与皮质活动的整合。然而，支持这些皮质波的神经回路元件仍有待完全定义。在这里，开发了一种新型的切向切片制备方法，该方法在使用聚焦电刺激后通过自发荧光成像评估时，在背侧皮质片上显示出传播波活动。通过谷氨酸非笼合的激光扫描光刺激对切片制备中的功能连接进行分析，揭示了在切片制备中缺乏短潜伏期、假定的单突触、长程连接（>300 μm）。这些结果建立了一种用于评估皮质动力学的新型切片制备方法，并支持了这样一种观点，即局部皮质元件之间的相互作用足以实现广泛传播的波活动。
