College of Horticulture and Plant Protection, Yangzhou University, No. 88 of Da Xue Nan Road, Hanjiang District, Yangzhou 225009, China.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
J Agric Food Chem. 2020 Mar 4;68(9):2623-2630. doi: 10.1021/acs.jafc.9b07342. Epub 2020 Feb 25.
is a typical xerophytic weed species with a cosmopolitan distribution. It is invasive and highly adaptable to diverse habitats and crops. Due to rice cropping-pattern changes, has become one of the main dominant grass weeds infecting direct-seeding paddy fields. A Chinese population has evolved multiple-herbicide resistance to cyhalofop-butyl and glyphosate. In this study, the multiple-resistance profile of to these two different types of herbicides and their resistance mechanisms were investigated. Whole-plant dose-response assays indicated that the multiple-herbicide-resistant (MHR) population exhibited 10.8-fold resistance to cyhalofop-butyl and 3.1-fold resistance to glyphosate compared with the susceptible (S) population. ACCase sequencing revealed that the Asp-2078-Gly mutation was strongly associated with resistance to cyhalofop-butyl. The MHR plants accumulated less shikimic acid than S plants at 4, 6, and 8 days after glyphosate treatment. In addition, no amino acid substitution in the gene was found in MHR plants. Further analysis revealed that the relative expression level of in MHR plants was 6-10-fold higher than that in S plants following glyphosate treatment, indicating that overexpression may contribute to the glyphosate resistance. Furthermore, the effectiveness of nine post-emergence herbicides against were evaluated, and one PPO inhibitor pyraclonil was identified as highly effective in controlling the S and MHR populations.
是一种典型的旱生杂草物种，具有世界性分布。它具有入侵性和高度适应性，可以适应多种生境和作物。由于水稻种植方式的改变，已成为侵染直播稻田的主要杂草之一。中国种群对氰氟草酯和草甘膦已经产生了多种除草剂抗性。在这项研究中，研究了对这两种不同类型除草剂的多抗性特征及其抗性机制。全株剂量反应试验表明，与敏感种群相比，多抗性种群对氰氟草酯的抗性提高了 10.8 倍，对草甘膦的抗性提高了 3.1 倍。ACCase 测序表明，Asp-2078-Gly 突变与对氰氟草酯的抗性密切相关。在草甘膦处理后 4、6 和 8 天，MHR 植株积累的莽草酸比 S 植株少。此外，在 MHR 植株中未发现 基因的氨基酸取代。进一步分析表明，在草甘膦处理后，MHR 植株中 的相对表达水平比 S 植株高 6-10 倍，表明 过表达可能有助于草甘膦抗性。此外，还评估了九种苗后除草剂对 和 MHR 的防治效果，发现一种 PPO 抑制剂吡唑草酮对 和 MHR 的防治效果非常好。
