Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan.
Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan; Division of Obstetrics and Gynecology, Support System for Community Medicine, Faculty of Medicine, Oita University, Oita, Japan.
J Reprod Immunol. 2020 Apr;138:103100. doi: 10.1016/j.jri.2020.103100. Epub 2020 Feb 6.
Endometriosis is a chronic inflammatory disease which is associated with aberrant chemokine expression. We have established a three-dimensional (3D) floating collagen gel culture of human endometriotic cyst stromal cells (ECSCs) as an in vitro model of early-stage fibrosis formation in endometriosis. We evaluated the gene expression profiles of 3D-cultured ECSCs using a gene expression microarray. We identified and confirmed with reverse transcription-polymerase chain reaction that mRNA levels of CXCL1, CXCL2, CXCL3, CXCL8, and CCL20 in 3D-cultured ECSCs were significantly higher than in 2D-cultured ECSCs. The protein levels of CXCL1, CXCL2, CXCL8, and CCL20 in the supernatant of 3D-cultured ECSCs were significantly higher than in 2D-cultured ECSCs. It has been suggested that the 3D-culture model of ECSCs is more suitable for in vitro endometriosis research than 2D-culture. This microarray data provides a new platform to identify the candidate genes involved in the pathogenesis of endometriosis which could be masked in conventional 2D-culture.
子宫内膜异位症是一种慢性炎症性疾病，与趋化因子表达异常有关。我们建立了人子宫内膜异位症囊肿基质细胞（ECSCs）的三维（3D）漂浮胶原凝胶培养物，作为子宫内膜异位症早期纤维化形成的体外模型。我们使用基因表达微阵列评估了 3D 培养的 ECSCs 的基因表达谱。我们通过逆转录-聚合酶链反应鉴定并证实，3D 培养的 ECSCs 中 CXCL1、CXCL2、CXCL3、CXCL8 和 CCL20 的 mRNA 水平明显高于 2D 培养的 ECSCs。3D 培养的 ECSCs 上清液中 CXCL1、CXCL2、CXCL8 和 CCL20 的蛋白水平明显高于 2D 培养的 ECSCs。有人认为，与 2D 培养相比，ECSCs 的 3D 培养模型更适合体外子宫内膜异位症研究。这些微阵列数据为鉴定参与子宫内膜异位症发病机制的候选基因提供了一个新的平台，这些基因在传统的 2D 培养中可能被掩盖。
