College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China.
College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China.
Plant Physiol Biochem. 2020 Apr;149:96-110. doi: 10.1016/j.plaphy.2020.01.036. Epub 2020 Jan 31.
NACs are one of the largest transcription factor families in plants and are involved in the response to abiotic stress. A new stress-responsive NAC transcription factor gene, LpNAC13, was isolated from Lilium pumilum bulbs. The expression of LpNAC13 was induced by drought, salt, cold and ABA treatments. LpNAC13 overexpressing plants were generated to explore the function of LpNAC13 in response to drought and salt stress. Overexpression of LpNAC13 in tobacco displayed a reduced drought tolerance but exhibited an enhanced salt tolerance. The LpNAC13 overexpression plants had decreased antioxidant enzyme activities, content of proline and chlorophyll, increased MDA content under drought condition, the results in the LpNAC13 plants under salt condition were opposite to those under drought condition. The seed germination and root length assays of overexpression of LpNAC13 showed decreased sensitivity to ABA. Functional analyses demonstrate that LpNAC13 plays opposite roles in drought and salt stress tolerance, acting as a negative regulator of drought response but as a positive regulator of salt response in tobacco.
NAC 是植物中最大的转录因子家族之一，参与非生物胁迫的响应。本研究从百合鳞茎中分离到一个新的胁迫响应 NAC 转录因子基因 LpNAC13。LpNAC13 的表达受干旱、盐、冷和 ABA 处理诱导。构建 LpNAC13 过表达植株，以探究 LpNAC13 在应对干旱和盐胁迫中的功能。LpNAC13 在烟草中的过表达导致耐旱性降低，但耐盐性增强。干旱条件下，LpNAC13 过表达植株的抗氧化酶活性、脯氨酸和叶绿素含量降低，MDA 含量增加，而在盐胁迫条件下的结果则与干旱条件下的结果相反。LpNAC13 过表达的种子萌发和根长分析显示对 ABA 的敏感性降低。功能分析表明，LpNAC13 在耐旱性和耐盐性中发挥相反的作用，作为干旱响应的负调节剂，但作为盐响应的正调节剂在烟草中发挥作用。
