A Comparison of the Variable J and Carbon-Isotopic Composition of Sugars Methods to Assess Mesophyll Conductance from the Leaf to the Canopy Scale in Drought-Stressed Cherry.

Conductance of CO across the mesophyll () frequently constrains photosynthesis () but cannot be measured directly. We examined of cherry ( L) subjected to severe drought using the variable method and carbon-isotopic composition (C) of sugars from the centre of the leaf, the leaf petiole sap, and sap from the largest branch. Depending upon the location of the plant from which sugars are sampled, may be estimated over scales ranging from a portion of the leaf to a canopy of leaves. Both the variable and C of sugars methods showed a reduction in as soil water availability declined. The C of sugars further from the source of their synthesis within the leaf did not correspond as closely to the diffusive and C-isotopic discrimination conditions reflected in the instantaneous measurement of gas exchange and chlorophyll-fluorescence utilised by the variable approach. Post-photosynthetic fractionation processes and/or the release of sugars from stored carbohydrates (previously fixed under different environmental and C-isotopic discrimination conditions) may reduce the efficacy of the C of sugars from leaf petiole and branch sap in estimating in a short-term study. Consideration should be given to the spatial and temporal scales at which is under observation in any experimental analysis.