Environmental & Occupational Physiology Laboratory, Haute Ecole Bruxelles-Brabant, 1180 Brussels, Belgium.
Intensive Care Department, Brugmann University Hospital, 1020 Brussels, Belgium.
Biomolecules. 2020 Feb 12;10(2):282. doi: 10.3390/biom10020282.
Oxygenation conditions are crucial for growth and tumor progression. Recent data suggests a decrease in cancer cell proliferation occurring after exposure to normobaric hyperoxia. Those changes are associated with fractal dimension. The purpose of this research was to study the impact of hyperoxia on apoptosis and morphology of leukemia cell lines. Two hematopoietic lymphoid cancer cell lines (a T-lymphoblastoid line, JURKAT and a B lymphoid line, CCRF-SB) were tested under conditions of normobaric hyperoxia (FiO > 60%, ± 18h) and compared to a standard group (FiO = 21%). We tested for apoptosis using a caspase-3 assay. Cell morphology was evaluated by cytospin, microphotography after coloration, and analysis by a fractal dimension calculation software. Our results showed that exposure of cell cultures to transient normobaric hyperoxia induced apoptosis (elevated caspase-3) as well as significant and precocious modifications in cell complexity, as highlighted by increased fractal dimensions in both cell lines. These features are associated with changes in structure (pycnotic nucleus and apoptosis) recorded by microscopic analysis. Such morphological alterations could be due to several molecular mechanisms and rearrangements in the cancer cell, leading to cell cycle inhibition and apoptosis as shown by caspase-3 activity. T cells seem less resistant to hyperoxia than B cells.
氧合条件对生长和肿瘤进展至关重要。最近的数据表明，暴露于常压高氧后，癌细胞增殖减少。这些变化与分形维数有关。本研究旨在研究高氧对白血病细胞系凋亡和形态的影响。两种造血淋巴癌细胞系（T 淋巴母细胞系 JURKAT 和 B 淋巴母细胞系 CCRF-SB）在常压高氧条件（FiO > 60%，± 18h）下进行了测试，并与标准组（FiO = 21%）进行了比较。我们使用 caspase-3 测定法检测细胞凋亡。通过细胞旋涂术、染色后的显微摄影和分形维数计算软件分析评估细胞形态。我们的结果表明，细胞培养物暴露于短暂的常压高氧会诱导细胞凋亡（caspase-3 升高），以及细胞复杂性的显著和早期改变，这两种细胞系的分形维数都增加了。这些特征与通过显微镜分析记录的结构变化（核固缩和细胞凋亡）有关。这种形态改变可能是由于癌细胞中的几种分子机制和重排导致细胞周期抑制和细胞凋亡，如 caspase-3 活性所示。T 细胞似乎比 B 细胞对高氧更敏感。
