Department of Life Sciences, Korea University, Seoul, 02841, Republic of Korea.
Department of Life Sciences, Korea University, Seoul, 02841, Republic of Korea.
Biochem Biophys Res Commun. 2020 Apr 16;524(4):951-956. doi: 10.1016/j.bbrc.2020.02.014. Epub 2020 Feb 12.
Innate immunity in plants relies on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located on the plant cell surface. CaLecRK-S.5, a pepper L-type lectin receptor kinase, has been shown to confer broad-spectrum resistance through priming activation. To further elucidate the molecular mechanism of CaLecRK-S.5, transgenic tobacco plants were generated in this study. Interestingly, hemizygous transgenic plants exhibited a high accumulation of CaLecRK-S.5, but this accumulation was completely abolished in homozygous transgenic plants by a cosuppression mechanism. Gain-of-function and loss-of-function analyses revealed that CaLecRK-S.5 plays a positive role in Phytophthora elicitin-mediated defense responses.
植物的先天免疫依赖于位于植物细胞表面的模式识别受体（PRRs）识别病原体相关的分子模式（PAMPs）。辣椒 L 型凝集素受体激酶 CaLecRK-S.5 通过引发激活被证明赋予广谱抗性。为了进一步阐明 CaLecRK-S.5 的分子机制，本研究生成了转基因烟草植物。有趣的是，半合子转基因植物表现出 CaLecRK-S.5 的高积累，但通过共抑制机制，纯合转基因植物中的这种积累完全被消除。功能获得和功能丧失分析表明，CaLecRK-S.5 在 Phytophthora elicitin 介导的防御反应中发挥积极作用。
