School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China.
State Key Laboratory of Non-Food Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning 530007, PR China.
Carbohydr Polym. 2020 Apr 1;233:115848. doi: 10.1016/j.carbpol.2020.115848. Epub 2020 Jan 9.
This study posed a novel strategy of interweaving carboxymethyl chitosan (CMCS) and HKUST-1 to build eco-friendly, recyclable, long-acting and intelligent antibacterial agent carrier of HKUST-1@CMCS. Combined characterizations revealed that the structure of HKUST-1@CMCS was destroyed step by step through different intensity of the stimulation of phosphate, thereby realizing intelligent release of antibacterial agent. The results showed that dimethyl fumarate-loaded HKUST-1@CMCS was much intelligent and long-acting (384 h, 0.04 M PBS) release performance than pure dimethyl fumarate, thus its inhibition zone diameters with and without stimulation of phosphate on S. aureus were 17.4 ± 0.1, 10.2 ± 0.7 mm at 7 d, respectively, while that of pure dimethyl fumarate had lost antibacterial activity at 2 d. With superior and long-acting antimicrobial activity, dimethyl fumarate-loaded HKUST-1@CMCS could effectively prolong the shelf life of strawberries as food packaging. Furthermore, HKUST-1@CMCS could easily regenerate, and regenerated HKSUT-1@CMCS still maintained intelligent response property at one cycle.
本研究提出了一种将羧甲基壳聚糖（CMCS）与 HKUST-1 交织在一起的新策略，构建了环保、可回收、长效和智能的 HKUST-1@CMCS 抗菌剂载体。综合表征表明，HKUST-1@CMCS 的结构通过不同强度的磷酸盐刺激逐步被破坏，从而实现了抗菌剂的智能释放。结果表明，负载马来酸二甲酯的 HKUST-1@CMCS 具有比纯马来酸二甲酯更高的智能和长效（384 h，0.04 M PBS）释放性能，因此其在磷酸盐刺激前后对金黄色葡萄球菌的抑菌圈直径分别为 17.4±0.1、10.2±0.7 mm，在 7 d 时，而纯马来酸二甲酯在 2 d 时已失去抗菌活性。负载马来酸二甲酯的 HKUST-1@CMCS 具有优异的长效抗菌活性，可有效延长草莓等食品的保质期。此外，HKUST-1@CMCS 易于再生，并且在一个循环中再生的 HKSUT-1@CMCS 仍保持智能响应特性。
