Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603 203, India.
Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.
Arch Microbiol. 2020 Jul;202(5):1127-1133. doi: 10.1007/s00203-020-01825-y. Epub 2020 Feb 14.
The human gut consists of > 1000 different bacterial species for the smooth functioning of the gut. In normal conditions, the antioxidant system present in cells minimize the effects of reactive oxygen species. Upon exposure to antibiotics, there is a rise in ROS level which induces oxidative stress to the cells, ultimately killing the cells. Two broad-spectrum antibiotics, streptomycin and gentamicin at a concentration of 50 µM and 25 µM, were treated with Bacillus subtilis SRMIST201901 (MN726522) and B. cereus SRMIST201902 (MN726923); the treatment reduced the cell counts. Considering the bacterial defense property which relies on the antioxidant mechanism, in this study, we have reported an antioxidant peptide (GM15) derived from glutathione oxidoreductase of spirulina (or called cyanobacteria) Arthrospira platensis (Ap) which reduced the intracellular oxidative stress. Cellular ROS detection was confirmed by fluorescent-associated cell sorting (FACS) using the DCFDA dye. Resazurin dye test also confirmed the activity of peptide on the growth of the Bacillus sp. Based on the results obtained, it was concluded that there was a significant (P < 0.05) reduction in the intracellular oxidative stress on treating with GM15 peptide. Overall, the study indicates the influence of antioxidant peptide on the intracellular oxidative stress, leading to the development of an antioxidant drug from glutathione oxidoreductase of A. platensis against oxidative-related stresses.
人类肠道中存在超过 1000 种不同的细菌物种，以确保肠道的正常运作。在正常情况下，细胞内的抗氧化系统可以最大限度地减少活性氧的影响。然而，当暴露于抗生素时，活性氧水平会升高，从而导致细胞氧化应激，最终导致细胞死亡。本研究使用两种广谱抗生素，链霉素和庆大霉素，浓度分别为 50µM 和 25µM，分别处理枯草芽孢杆菌 SRMIST201901（MN726522）和蜡样芽孢杆菌 SRMIST201902（MN726923）；结果显示，这种处理降低了细胞数量。考虑到细菌防御特性依赖于抗氧化机制，我们从螺旋藻（或蓝藻）钝顶螺旋藻的谷胱甘肽氧化还原酶中报告了一种抗氧化肽（GM15），该肽可减轻细胞内的氧化应激。通过使用 DCFDA 染料的荧光相关细胞分选（FACS）确认了细胞内 ROS 的检测。Resazurin 染料试验也证实了该肽对芽孢杆菌生长的活性。基于获得的结果，我们得出结论，在用 GM15 肽处理后，细胞内氧化应激显著降低（P<0.05）。总的来说，该研究表明抗氧化肽对细胞内氧化应激的影响，从而从螺旋藻的谷胱甘肽氧化还原酶中开发出一种针对氧化相关应激的抗氧化药物。
