College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun, 130118, China.
Biomaker Technologies Corporation, Beijing, China.
Chemosphere. 2020 Apr;245:125613. doi: 10.1016/j.chemosphere.2019.125613. Epub 2019 Dec 10.
The heavy metal cadmium (Cd) is a hazardous pollutant known to exert various toxic effects and other sublethal to lethal effects on aquatic organisms, and can be commonly found in environment. The genus Bacillus was one of dominant probiotics, which was commonly used in aquaculture. The aim of this study was to evaluate the effects of dietary administration of Bacillus cereus (B. cereus) on bioaccumulation, oxidative stress and intestinal microbiota of Carassius auratus gibelio (C. gibelio) after Cd exposure. Fish were exposed for 4 weeks to waterborne Cd at 0, 1 and 2 mg/L and/or dietary B. cereus at 10 cfu/g. At 2 and 4 weeks, the fish were sampled and bioaccumulation, antioxidant activity and intestinal microbiota were assessed. Waterborne Cd exposure caused marked alterations in the composition of the microbiota. Dietary supplementation with B. cereus can reverse the changes in the composition of intestinal microbiota in Cd exposure and increase the abundance of Bacteroides, Akkermansia, Cetobacterium in the 0 and 1 mg/L Cd. The results obtained indicate that B. cereus can provide a significant protective effect on the toxicity of cadmium by inhibiting alterations in the levels of bioaccumulation and antioxidant enzyme including Superoxide dismutase (SOD), catalase (CAT), total antioxidant (T-AOC), glutathione (GSH), malonaldehyde (MDA) and Glutathione-S-transferase (GST). The present findings imply that dietary supplement of B. cereus can effectively protect C.gibelio to combat cadmium toxicity.
重金属镉（Cd）是一种有害污染物，已知其对水生生物具有多种毒性作用和其他亚致死到致死作用，并且在环境中很常见。芽孢杆菌属是一种优势益生菌，常用于水产养殖。本研究旨在评估日粮中添加蜡样芽孢杆菌（B. cereus）对 Cd 暴露后鲫（Carassius auratus gibelio）生物蓄积、氧化应激和肠道微生物群的影响。鱼在水中暴露于 0、1 和 2mg/L 的 Cd 以及/或日粮中 10cfu/g 的 B. cereus 4 周。在 2 和 4 周时，取样鱼并评估生物蓄积、抗氧化活性和肠道微生物群。水相 Cd 暴露导致微生物群组成发生明显改变。日粮补充 B. cereus 可以逆转 Cd 暴露对肠道微生物群组成的改变，并增加 0 和 1mg/L Cd 中拟杆菌属、阿克曼氏菌属、鲸杆菌属的丰度。研究结果表明，B. cereus 通过抑制生物蓄积和抗氧化酶（包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、总抗氧化（T-AOC）、谷胱甘肽（GSH）、丙二醛（MDA）和谷胱甘肽-S-转移酶（GST）水平的变化，对镉毒性具有显著的保护作用。这些发现表明，日粮补充 B. cereus 可以有效保护鲫对抗镉毒性。
