Department of Physiology, Institute of Biosciences, Sao Paulo State University-UNESP, Botucatu, SP, Brazil.
Department of Anatomy, Institute of Biosciences, Sao Paulo State University-UNESP, Botucatu, SP, Brazil.
Respir Physiol Neurobiol. 2020 May;276:103368. doi: 10.1016/j.resp.2020.103368. Epub 2020 Feb 22.
The Lateral Hypothalamus/Perifornical Area (LH/PFA) has been shown to be involved with the hypercapnic ventilatory response, in a state-dependent manner. We have demonstrated that purinergic signaling through ATP in the LH/PFA has an excitatory effect in ventilatory response to CO in awake rats in the dark phase of the diurnal cycle, but it is unknown whether the ATP metabolite adenosine, acting in the LH/PFA, modulates the ventilatory responses to hypercapnia. Here, we studied the effects of the microdialysis of adenosine (A1/A2 adenosine receptors agonist; 17 mM) and an A1 receptor antagonist (DPCPX; 0.1 mM) into the LH/PFA of conscious rats on ventilation in room air and in 7% CO during the light and the dark phases of the diurnal cycle. The microdialysis of adenosine and DPCPX caused no change in the CO ventilatory responses of rats during wakefulness or NREM sleep in either the dark or light period. Our data suggest that adenosine in the LH/PFA does not contribute to the hypercapnic ventilatory response in conscious rats.
外侧下丘脑/peri 穹窿区域（LH/PFA）已被证明与高碳酸血症通气反应有关，具有状态依赖性。我们已经证明，在白天周期的暗相期间，在清醒大鼠中，LH/PFA 中的嘌呤能信号通过 ATP 具有对 CO 通气反应的兴奋作用，但尚不清楚 LH/PFA 中的 ATP 代谢物腺苷是否通过作用来调节高碳酸血症的通气反应。在这里，我们研究了在白天周期的暗相和亮相期间，将腺苷（A1/A2 腺苷受体激动剂；17mM）和 A1 受体拮抗剂（DPCPX；0.1mM）微透析到清醒大鼠的 LH/PFA 中对通气的影响在空气和 7%CO 中的作用。在白天或黑夜期间，微透析腺苷和 DPCPX 并未引起大鼠在觉醒或 NREM 睡眠期间的 CO 通气反应发生变化。我们的数据表明，LH/PFA 中的腺苷不会导致清醒大鼠的高碳酸血症通气反应。
