Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, China.
Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, China.
Mol Cell Endocrinol. 2020 Apr 15;506:110759. doi: 10.1016/j.mce.2020.110759. Epub 2020 Feb 13.
To investigate the role of miR-411-5p and miR-434-3p in osteoblast differentiation in particulate-induced osteolysis.
A mouse model of osteolysis and an in vitro osteolysis model were constructed. The expressions of molecules were detected using qRT-PCR and western blot. Alkaline phosphatase (ALP) activity was measured using the ALP Assay Kit, and the bone mineralization was measured using alizarin red staining.
The expression of miR-411-5p and miR-434-3p was decreased in osteolysis mice and UHMWPE-induced mMSCs, while GATA4 protein expression was increased. Over-expression of miR-411-5p and miR-434-3p up-regulated the expressions of osteoblast gene markers, enhanced the ALP activity, promoted the bone mineralization of mesenchymal stem cells. In addition, miR-411-5p and miR-434-3p could target GATA4, and miR-411-5p/434-3p affected the expressions of osteoblast gene markers through GATA4 in vitro and in vivo.
Overexpression of miR-411-5p and miR-434-3p promoted the osteoblast differentiation by inhibiting GATA4 expression.
探讨 miR-411-5p 和 miR-434-3p 在颗粒诱导的破骨细胞分化中的作用。
构建小鼠溶骨模型和体外溶骨模型。采用 qRT-PCR 和 Western blot 检测分子表达。使用碱性磷酸酶（ALP）测定试剂盒测定 ALP 活性，使用茜素红染色测定骨矿化。
miR-411-5p 和 miR-434-3p 在溶骨小鼠和 UHMWPE 诱导的 mMSCs 中的表达降低，而 GATA4 蛋白表达增加。过表达 miR-411-5p 和 miR-434-3p 上调成骨细胞基因标志物的表达，增强 ALP 活性，促进间充质干细胞的骨矿化。此外，miR-411-5p 和 miR-434-3p 可以靶向 GATA4，并且 miR-411-5p/434-3p 通过体外和体内的 GATA4 影响成骨细胞基因标志物的表达。
过表达 miR-411-5p 和 miR-434-3p 通过抑制 GATA4 表达促进成骨细胞分化。
