甘草次酸对实验性自身免疫性脑脊髓炎小鼠模型的作用。
The Effects of Carbenoxolone against Experimental Autoimmune Encephalomyelitis in a Mouse Model.
机构信息
Department of Neurology, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China,
Department of Orthopaedics, Traditional Chinese Medicine Hospital of Wuhu City, The Affiliated Hospital of Anhui University of Chinese Medicine, Wuhu, China.
出版信息
Neuroimmunomodulation. 2020;27(1):19-27. doi: 10.1159/000505333. Epub 2020 Feb 14.
INTRODUCTION
Multiple sclerosis (MS) is a complex demyelinating disease involving central nervous system (CNS). It is still a challenge to secure an effective therapeutic strategy against this disease. Carbenoxolone (CBX) is a derivative of glycyrrhetinic acid, which is widely used in brain research for its gap-junction inhibition effects. Many researchers have observed CBX-mediated suppression of CNS inflammation in their studies.
OBJECTIVE
We want to further examine its anti-inflammation effects in CNS demyelinating disease like MS.
METHODS
Thus, our study applied an experimental autoimmune encephalomyelitis (EAE) mouse model and examined the effects of CBX on it.
RESULTS AND CONCLUSIONS
We found that CBX significantly reversed the EAE severity and pathology in EAE. IL-17-secreting and IFN-γ-secreting CD4+ T lymphocytes were remarkably lower in the spleen of CBX-treated mice. Production of IL-23 and IL-17 from cortex in EAE animals was markedly reduced by CBX. Furthermore, CBX treatment increased the expression of brain-derived neurotrophic factor. This study provides evidence for the protective role of CBX against EAE.
简介
多发性硬化症（MS）是一种累及中枢神经系统（CNS）的复杂脱髓鞘疾病。目前，针对这种疾病仍缺乏有效的治疗策略。甘草次酸的衍生物卡波氯铵（CBX）因具有缝隙连接抑制作用，在脑科学研究中得到了广泛的应用。许多研究人员观察到 CBX 可抑制中枢神经系统炎症。
目的
我们希望进一步研究 CBX 在多发性硬化症等脱髓鞘性中枢神经系统疾病中的抗炎作用。
方法
为此，我们应用实验性自身免疫性脑脊髓炎（EAE）小鼠模型来研究 CBX 的作用。
结果与结论
结果发现，CBX 可显著减轻 EAE 的严重程度和病理学改变。与对照组相比，CBX 处理组小鼠脾脏中分泌白细胞介素 17（IL-17）和干扰素γ（IFN-γ）的 CD4+T 淋巴细胞显著减少。CBX 还可显著降低 EAE 动物皮质中白细胞介素 23（IL-23）和白细胞介素 17（IL-17）的产生。此外，CBX 治疗可增加脑源性神经营养因子的表达。本研究为 CBX 对 EAE 的保护作用提供了证据。
Zotero 插件