开发含有氯化十六烷基吡啶蒙脱石的组织调理剂作为新型抗菌剂：抗菌活性和生物相容性的初步研究。

Development of tissue conditioner containing cetylpyridinium chloride montmorillonite as new antimicrobial agent: Pilot study on antimicrobial activity and biocompatibility.

机构信息

Oral Functional Prosthodontics, Department of Oral Functional Science, Faculty and Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8586, Japan.

Oral Molecular Microbiology, Department of Oral Pathobiological Science, Faculty and Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8586, Japan.

出版信息

J Prosthodont Res. 2020 Oct;64(4):436-443. doi: 10.1016/j.jpor.2019.12.002. Epub 2020 Feb 13.

DOI:10.1016/j.jpor.2019.12.002

PMID:32063532

Abstract

PURPOSE

The mechanical properties, antimicrobial activity, and biocompatibility of a novel antimicrobial tissue conditioner containing cetylpyridinium chloride with montmorillonite (CPC-Mont) were evaluated.

METHODS

To examine the mechanical properties of the novel material, hardness, consistency, and penetration tests were conducted. Antimicrobial activity against Candida albicans (C. albicans) and Staphylococcus aureus (S. aureus) was evaluated. Cell viabilities of fibroblasts and epithelial cells using eluates from materials were measured to evaluate cytotoxicity. In addition, to assess tissue response, animal experiments were conducted.

RESULTS

The hardness test results were similar to those of other commercially available materials. The novel tissue conditioner showed good antimicrobial activity against C. albicans and S. aureus compared with other materials. This effect was sustained for a week for C. albicans. In the case of S. aureus, microbial growth was suppressed for up to 3 weeks. Cell viability of the novel material for the eluate at 1 day was significantly less than those of other material for both cells. However, the cell viability at 7 days showed no significant difference. Animal experiments demonstrated that inflammatory responses around materials were not observed on the oral mucosa as other material.

CONCLUSION

Within the limitations of this in vitro and in vivo study, the results suggest that the newly developed tissue conditioner containing CPC-Mont has not only excellent antimicrobial properties, but also the same mechanical properties and biocompatibility as tissue conditioners on the market.

摘要

目的

评估含有氯化十六烷基吡啶和蒙脱石的新型抗菌组织调节剂（CPC-Mont）的机械性能、抗菌活性和生物相容性。

方法

为了研究新型材料的机械性能，进行了硬度、稠度和穿透测试。评估了该材料对白色念珠菌（C. albicans）和金黄色葡萄球菌（S. aureus）的抗菌活性。使用材料浸提液测量成纤维细胞和上皮细胞的细胞活力，以评估细胞毒性。此外，进行了动物实验以评估组织反应。

结果

硬度测试结果与其他市售材料相似。与其他材料相比，新型组织调节剂对 C. albicans 和 S. aureus 表现出良好的抗菌活性。这种效果对 C. albicans 持续了一周。在 S. aureus 的情况下，微生物生长抑制长达 3 周。新型材料浸提液在第 1 天的细胞活力明显低于其他材料的细胞活力。然而，第 7 天的细胞活力没有显著差异。动物实验表明，在口腔黏膜上，新型材料周围没有观察到其他材料那样的炎症反应。