Okazaki Yohei, Nakamori Kiichi, Yao Chenmin, Ahmed Mohammed H, Mercelis Benjamin, Nagaoka Noriyuki, Maruo Yukinori, Yoshida Yasuhiro, Abe Yasuhiko, Van Meerbeek Bart, Yoshihara Kumiko
Department of Oral Health Sciences, BIOMAT, KU Leuven, 3000 Leuven, Belgium.
Department of Advanced Prosthodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Materials (Basel). 2024 Sep 4;17(17):4368. doi: 10.3390/ma17174368.
Oral bacteria cause tooth caries and periodontal disease. Much research is being conducted to prevent both major oral diseases by rendering dental materials' antimicrobial potential. However, such antimicrobial materials are regarded as 'combination' products and face high hurdles for regulatory approval. We loaded inorganic montmorillonite with the antimicrobial agent cetylpyridinium chloride, referred to below as 'CPC-Mont'. CPC-Mont particles in a 1, 3 and 5 wt% concentration were added to the considered gold-standard self-etch adhesive Clearfil SE Bond 2 ('CSE2'; Kuraray Noritake) to render its antibacterial potential (CSE2 without CPC-Mont served as control). Besides measuring (immediate) bonding effectiveness and (aged) bond durability to dentin, the antibacterial activity against and the polymerization-conversion rate was assessed. Immediate and aged bond strength was not affected by 1 and 3 wt% CPC-Mont addition, while 5 wt% CPC-Mont significantly lowered bond strength and bond durability. The higher the concentration of the antimicrobial material added, the stronger the antimicrobial activity. Polymerization conversion was not affected by the CPC-Mont addition in any of the three concentrations. Hence, adding 3 wt% CPC-Mont to the two-step self-etch adhesive rendered additional antimicrobial potential on top of its primary bonding function.
口腔细菌会导致龋齿和牙周疾病。目前正在进行大量研究，通过赋予牙科材料抗菌潜力来预防这两种主要的口腔疾病。然而，这类抗菌材料被视为“组合”产品，面临着监管审批的高门槛。我们将抗菌剂氯化十六烷基吡啶负载到无机蒙脱石上，以下简称“CPC-蒙脱石”。将浓度为1%、3%和5%的CPC-蒙脱石颗粒添加到被视为金标准的自酸蚀粘结剂Clearfil SE Bond 2（“CSE2”；可乐丽诺瑞特公司）中，以赋予其抗菌潜力（不含CPC-蒙脱石的CSE2作为对照）。除了测量（即时）粘结效果和（老化后）与牙本质的粘结耐久性外，还评估了对……的抗菌活性和聚合转化率。添加1%和3%的CPC-蒙脱石对即时和老化粘结强度没有影响，而添加5%的CPC-蒙脱石会显著降低粘结强度和粘结耐久性。添加的抗菌材料浓度越高，抗菌活性越强。在这三种浓度中的任何一种情况下，CPC-蒙脱石的添加都不会影响聚合转化率。因此，在两步自酸蚀粘结剂中添加3%的CPC-蒙脱石，在其主要粘结功能之上还赋予了额外的抗菌潜力。
