Liu Junli, Wang Fangfang, Du Liuyang, Li Juan, Yu Tianqi, Jin Yulan, Yan Yan, Zhou Jiyong, Gu Jinyan
MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety, Institute of Immunology, Nanjing Agricultural University, Nanjing, China.
MOA Key Laboratory of Animal Virology, Department of Veterinary Medicine, Zhejiang University, Hangzhou, China.
Front Microbiol. 2020 Jan 28;10:3036. doi: 10.3389/fmicb.2019.03036. eCollection 2019.
Porcine delta coronavirus (PDCoV) is a novel emerging enterocytetropic virus causing diarrhea, vomiting, dehydration, and mortality in suckling piglets. Long non-coding RNAs (lncRNAs) are known to be important regulators during virus infection. Here, we describe a comprehensive transcriptome profile of lncRNA in PDCoV-infected swine testicular (ST) cells. In total, 1,308 annotated and 1,190 novel lncRNA candidate sequences were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these lncRNAs might be involved in numerous biological processes. Clustering analysis of differentially expressed lncRNAs showed that 454 annotated and 376 novel lncRNAs were regulated after PDCoV infection. Furthermore, we constructed a lncRNA-protein-coding gene co-expression interaction network. The KEGG analysis of the co-expressed genes showed that these differentially expressed lncRNAs were enriched in pathways related to metabolism and TNF signaling. Our study provided comprehensive information about lncRNAs that would be a useful resource for studying the pathogenesis of and designing antiviral therapy for PDCoV infection.
猪德尔塔冠状病毒（PDCoV）是一种新出现的嗜肠细胞病毒，可导致哺乳仔猪腹泻、呕吐、脱水和死亡。已知长链非编码RNA（lncRNA）在病毒感染过程中是重要的调节因子。在此，我们描述了PDCoV感染的猪睾丸（ST）细胞中lncRNA的全面转录组概况。总共鉴定出1308个注释的和1190个新的lncRNA候选序列。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，这些lncRNA可能参与众多生物学过程。差异表达lncRNA的聚类分析显示，PDCoV感染后454个注释的和376个新的lncRNA受到调控。此外，我们构建了一个lncRNA-蛋白质编码基因共表达相互作用网络。对共表达基因的KEGG分析表明，这些差异表达的lncRNA富集在与代谢和TNF信号相关的通路中。我们的研究提供了有关lncRNA的全面信息，这将是研究PDCoV感染的发病机制和设计抗病毒治疗的有用资源。
