Xiao Yanyan, Liu Yong, Qin Xiaosong
Department of Clinical Laboratory, Shengjing Hospital of China Medical University, Shenyang, China.
Can J Infect Dis Med Microbiol. 2020 Jan 21;2020:8753284. doi: 10.1155/2020/8753284. eCollection 2020.
The aim of this study was to evaluate the clinical application of three methods for detecting in fecal samples.
One hundred and fifty fecal specimens were collected and tested for using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay. The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay.
Of 150 specimens, 26 carried both A and B toxin genes, and none of the samples were positive for the binary toxin gene. Toxin-producing using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay. The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay. using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay. The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay.
The VIDAS GDH assay is useful for initial screening of using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay. The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay.
本研究旨在评估三种检测粪便样本中[具体物质]的方法的临床应用。
收集了150份粪便标本，并使用三种方法进行检测：（1）产毒培养（TC）；（2）VIDAS酶免疫测定（EIA）：使用VIDAS谷氨酸脱氢酶（GDH）测定和毒素A/B测定来检测GDH抗原和A/B毒素；（3）GeneXpert PCR测定。以产毒培养作为参考来评估VIDAS EIA和GeneXpert PCR测定的性能。
在150份标本中，26份同时携带A和B毒素基因，且没有样本的二元毒素基因呈阳性。使用三种方法检测产毒[具体物质]：（1）产毒培养（TC）；（2）VIDAS酶免疫测定（EIA）：使用VIDAS谷氨酸脱氢酶（GDH）测定和毒素A/B测定来检测GDH抗原和A/B毒素；（3）GeneXpert PCR测定。以产毒培养作为参考来评估VIDAS EIA和GeneXpert PCR测定的性能。使用三种方法检测产毒[具体物质]：（1）产毒培养（TC）；（2）VIDAS酶免疫测定（EIA）：使用VIDAS谷氨酸脱氢酶（GDH）测定和毒素A/B测定来检测GDH抗原和A/B毒素；（3）GeneXpert PCR测定。以产毒培养作为参考来评估VIDAS EIA和GeneXpert PCR测定的性能。
VIDAS GDH测定对于使用三种方法检测[具体物质]的初步筛查有用：（1）产毒培养（TC）；（2）VIDAS酶免疫测定（EIA）：使用VIDAS谷氨酸脱氢酶（GDH）测定和毒素A/B测定来检测GDH抗原和A/B毒素；（3）GeneXpert PCR测定。以产毒培养作为参考来评估VIDAS EIA和GeneXpert PCR测定的性能。
需注意，原文中部分内容重复，翻译时尽量保持了原文的结构和表述。同时，“[具体物质]”表示原文中未明确给出的物质名称。
